Detailed information    

experimental Experimentally validated

Overview

Name   lrpC   Type   Machinery gene
Locus tag   BSU_04250 Genome accession   NC_000964
Coordinates   476059..476493 (+) Length   144 a.a.
NCBI ID   NP_388306.1    Uniprot ID   P96582
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination   
Homologous recombination

Function

LrpC binds at different sites within the same DNA molecule promoting intramolecular ligation. When bound to separate molecules, it promotes intermolecular ligation, and joint molecule formation between a circular ssDNA and a homologous ssDNA-tailed linear dsDNA.


Genomic Context

Location: 471059..481493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_04190 (BSU04190) ydaD 471709..472569 (+) 861 NP_388300.1 putative dehydrogenase -
  BSU_04200 (BSU04200) lyxE 472585..473088 (+) 504 NP_388301.1 D-lyxose ketol isomerase -
  BSU_04210 (BSU04210) ydaF 473174..473725 (+) 552 NP_388302.1 putative ribosomal protein N-acetyltransferase -
  BSU_04220 (BSU04220) ydaG 473803..474225 (+) 423 NP_388303.1 putative general stress protein -
  BSU_04230 (BSU04230) amj 474731..475540 (+) 810 NP_388304.1 lipid II flippase -
  BSU_04240 (BSU04240) ydzA 475584..475874 (-) 291 NP_388305.1 hypothetical protein -
  BSU_04250 (BSU04250) lrpC 476059..476493 (+) 435 NP_388306.1 transcriptional regulator (Lrp/AsnC family) Machinery gene
  BSU_04260 (BSU04260) topB 476558..478741 (+) 2184 NP_388307.1 DNA topoisomerase III -
  BSU_04270 (BSU04270) epsJ 478944..480032 (+) 1089 NP_388308.1 glycosyl hydrolase lipoprotein -
  BSU_04280 (BSU04280) epsK 480013..480864 (+) 852 NP_388309.1 cyclic-di-GMP receptor -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16450.03 Da        Isoelectric Point: 7.7037

>NTDB_id=645 BSU_04250 NP_388306.1 476059..476493(+) (lrpC) [Bacillus subtilis subsp. subtilis str. 168]
MKLDQIDLNIIEELKKDSRLSMRELGRKIKLSPPSVTERVRQLESFGIIKQYTLEVDQKKLGLPVSCIVEATVKNADYER
FKSYIQTLPNIEFCYRIAGAACYMLKINAESLEAVEDFINKTSPYAQTVTHVIFSEIDTKNGRG

Nucleotide


Download         Length: 435 bp        

>NTDB_id=645 BSU_04250 NP_388306.1 476059..476493(+) (lrpC) [Bacillus subtilis subsp. subtilis str. 168]
ATGAAACTTGACCAGATTGATCTGAATATCATTGAGGAGCTGAAGAAGGACAGCCGTTTGTCGATGAGGGAATTAGGCAG
AAAAATTAAGCTGTCGCCTCCATCTGTAACAGAACGGGTAAGACAGCTTGAATCGTTTGGCATCATCAAGCAATACACGC
TGGAGGTCGACCAGAAAAAACTGGGGCTTCCCGTTTCCTGCATTGTGGAAGCAACCGTTAAAAACGCGGATTATGAGCGG
TTCAAAAGCTATATTCAAACATTGCCGAATATTGAATTTTGCTACCGGATTGCGGGTGCAGCCTGCTATATGCTGAAAAT
CAATGCCGAAAGCCTCGAAGCGGTAGAAGATTTCATTAACAAAACATCGCCCTACGCGCAAACCGTCACTCACGTCATTT
TCTCAGAAATTGACACGAAAAACGGGCGCGGTTAG

Domains

Predicted by InterProScan.

Lrp/AsnC ligand binding domain (AsnC_trans_reg)

(70-135)

Winged helix-turn-helix DNA-binding (HTH_24)

(3-50)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 2CFX

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Gema López-Torrejón et al. (2006) Role of LrpC from Bacillus subtilis in DNA transactions during DNA repair and recombination. Nucleic Acids Research 34(1):120-9. [PMID: 16407330]