Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L0Y82_RS22410 Genome accession   NZ_CP090673
Coordinates   2584444..2585949 (+) Length   501 a.a.
NCBI ID   WP_006398255.1    Uniprot ID   -
Organism   Burkholderia multivorans strain 2020Y71628512IV     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2579444..2590949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Y82_RS22385 (L0Y82_22390) kdsD 2580695..2581678 (+) 984 WP_006406620.1 arabinose 5-phosphate isomerase KdsD -
  L0Y82_RS22390 (L0Y82_22395) - 2581678..2582214 (+) 537 WP_006406621.1 HAD family hydrolase -
  L0Y82_RS22395 (L0Y82_22400) lptC 2582218..2582832 (+) 615 WP_006398258.1 LPS export ABC transporter periplasmic protein LptC -
  L0Y82_RS22400 (L0Y82_22405) lptA 2582853..2583518 (+) 666 WP_006398257.1 lipopolysaccharide transport periplasmic protein LptA -
  L0Y82_RS22405 (L0Y82_22410) lptB 2583515..2584291 (+) 777 WP_006398256.1 LPS export ABC transporter ATP-binding protein -
  L0Y82_RS22410 (L0Y82_22415) rpoN/rpoN1 2584444..2585949 (+) 1506 WP_006398255.1 RNA polymerase factor sigma-54 Machinery gene
  L0Y82_RS22415 (L0Y82_22420) raiA 2586108..2586467 (+) 360 WP_006398254.1 ribosome-associated translation inhibitor RaiA -
  L0Y82_RS22420 (L0Y82_22425) - 2586662..2587174 (+) 513 WP_035488861.1 PTS sugar transporter subunit IIA -
  L0Y82_RS22425 (L0Y82_22430) hprK 2587303..2588271 (+) 969 WP_249476193.1 HPr(Ser) kinase/phosphatase -
  L0Y82_RS22430 (L0Y82_22435) rapZ 2588348..2589256 (+) 909 WP_006409809.1 RNase adapter RapZ -
  L0Y82_RS22435 (L0Y82_22440) - 2589284..2589919 (+) 636 WP_006406624.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54746.94 Da        Isoelectric Point: 4.8740

>NTDB_id=644304 L0Y82_RS22410 WP_006398255.1 2584444..2585949(+) (rpoN/rpoN1) [Burkholderia multivorans strain 2020Y71628512IV]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPVSNGEPAI
ANGNGQSSDRAERDEPAGADEYDGYAAGDASDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
KLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=644304 L0Y82_RS22410 WP_006398255.1 2584444..2585949(+) (rpoN/rpoN1) [Burkholderia multivorans strain 2020Y71628512IV]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGTCCGCTGCGCGTTGCGGCGGACGGCTCGCTGATCGCGCAGACGCCGCCCGTGTCGAACGGCGAGCCGGCCATC
GCGAACGGCAACGGCCAGTCGAGCGATCGTGCGGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTACGCGGC
CGGCGACGCGAGCGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCCTCCGACGACGACGATCTGC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGATCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCAAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCATCGCTCGACGAGGTGCT
CGCCGATCTGCCGCCCGAACTCGAAGTCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGTTCGGCATCGGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAACATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGGTTGCGCAAGCATCTGAAGGCCAC
CGACGACGCGCTGCGCGACGCACACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCCTACGGCAAGGCCGAAG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAAGTCGGCCAGGGCTGGCAGGCCGAACTCAATCCCGAGGTCGTGCCG
AAGCTGCGCATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTGAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTCATCAAGAACATCCAGCAGCGGTTCGACACGATCCTGCGCGTCGCGCAGGCGATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACGGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCCAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695