Detailed information    

experimental Experimentally validated

Overview

Name   sbcE   Type   Machinery gene
Locus tag   BSU_09920 Genome accession   NC_000964
Coordinates   1066077..1068968 (+) Length   963 a.a.
NCBI ID   NP_388873.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination   
Homologous recombination

Function

SbcE plays a role during transformation in competent cells and in DNA double-strand break (DSB) repair.


Genomic Context

Location: 1061077..1073968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_09880 (BSU09880) yhaR 1061491..1062258 (+) 768 NP_388869.3 putative dehydratase / isomerase -
  BSU_09889 (BSU09889) yhzD 1062279..1062464 (-) 186 YP_003097698.1 hypothetical protein -
  BSU_09890 (BSU09890) yhaQ 1062591..1063487 (+) 897 NP_388870.2 Na+-dependent efflux ABC transporter (ATP-binding protein) -
  BSU_09900 (BSU09900) yhaP 1063480..1064739 (+) 1260 NP_388871.2 Na+-dependent exporter (ABC permease) -
  BSU_09910 (BSU09910) yhaO 1064846..1066072 (+) 1227 NP_388872.1 putative DNA repair exonuclease -
  BSU_09920 (BSU09920) sbcE 1066077..1068968 (+) 2892 NP_388873.1 ATPase involved in DNA double strand break repair and recombination Machinery gene
  BSU_09930 (BSU09930) yhaM 1069042..1069986 (+) 945 NP_388874.1 3'-5' exonuclease -
  BSU_09940 (BSU09940) yhaL 1070111..1070323 (+) 213 NP_388875.1 sporulation factor -
  BSU_09950 (BSU09950) prsA 1070364..1071242 (-) 879 NP_388876.1 molecular chaperone lipoprotein -
  BSU_09958 (BSU09958) sscA 1071402..1071488 (+) 87 YP_003097699.1 spore assembly and germination protein -
  BSU_09959 (BSU09959) sscB 1071613..1071699 (+) 87 YP_009513948.1 spore and germination protein -
  BSU_09965 (BSU09965) yhaJ 1072042..1072560 (-) 519 NP_388878.2 putative membrane enzyme -
  BSU_09980 (BSU09980) yhaI 1072768..1073109 (+) 342 NP_388879.1 hypothetical protein -
  BSU_09990 (BSU09990) scoC 1073106..1073717 (-) 612 NP_388880.1 transcriptional regulator of extracellular protease production, sporulation and bacilysin production (MarR family) -

Sequence

Protein


Download         Length: 963 a.a.        Molecular weight: 111159.16 Da        Isoelectric Point: 6.1206

>NTDB_id=642 BSU_09920 NP_388873.1 1066077..1068968(+) (sbcE) [Bacillus subtilis subsp. subtilis str. 168]
MTALRIISLHIYQYGKFSNRTFDFSASPVQVIYGLNEAGKTTMMSFIESMLFGFPKTKKYEPKTGGVYGGVLEAEHPEYG
VLKIERTKGTAEKLSVYTEKGEVKQGDVLKQLFQGTDRSLYKAIYSFDVFGLQEIHAFNRDKIGEFLLFSSLFGAEAVSK
LDSRLTKESERLYKPNGRNPQLNQELETLKQLAVKLKQAEAEEAGYHQLLEEKRTLEARLAAAETELKETAGHIRMIEGA
IERKPLLNEKATLEQVIAEFPEHAGQFPADGLHQLEKYESHLHPKSAQLEALRVKMAELDKQRQKLIPDKELLAKETLIQ
ELSAAFHMYQSWGEQLAAIQAQLRQTSAQTAAGLEQLNKTDENELLNMNTSYDYEWQLQQAVQQYVQARDRKRQLDETFE
LARQELEDAEKAVRAASSAILENSQRKDKEAALRAYDETQGQHQEQAKLREQLTFFERQQAKQKKTVIAAGMLFIVLFSL
LQQWIPAISFGAALIVYWLVSGKSSSRNSRETRQPMTDISPAEAEMLREALWEDDRNKQHLLTQRAALQQKEAAYERVIQ
QFEQWEAEMAPSFTQVERFMNELGFKEDPSFLLDAYSLMKDVKKEVKKKHELTIEAGRLKKHRRTFEERVSMLLPVNQSQ
DISISDALHTLRKNIEREKEIEKQKKEIETDIHYTKEQMLELEQEIQYFHAQIEQLFAAAAAKDRDAFFAIAAISRQLKD
TENKLHHVNAQLQGGYPEELELADSNTLSELKDKQFVENERKERLTEEIEQLRSQIALLSVKQEQLEASGMVSDLKLQTE
MQKERVKETAKKWASIQMVKQVIRNKLERHKKIELPRLLETAGEFFRPLTDGNYQTIYFSETDDSIMVMHRDGTVYHAEE
LSQGTCEQLYTAIRFALAVTRQGESKLPFQLDDSFVHFDQERLKRVLHVLYDLSEGGRQILYFTCHEHVKDAFHSSQIIH
LVS

Nucleotide


Download         Length: 2892 bp        

>NTDB_id=642 BSU_09920 NP_388873.1 1066077..1068968(+) (sbcE) [Bacillus subtilis subsp. subtilis str. 168]
GTGACAGCTTTGCGAATTATATCTTTACATATCTACCAATACGGAAAATTTTCCAATCGGACCTTTGACTTTTCCGCCTC
TCCTGTCCAAGTCATCTACGGATTAAATGAGGCAGGAAAAACGACGATGATGTCATTCATTGAAAGCATGCTGTTCGGTT
TTCCGAAAACGAAAAAGTATGAACCGAAAACAGGCGGTGTCTACGGCGGTGTTCTTGAAGCTGAACATCCTGAGTATGGG
GTGCTGAAGATCGAACGGACGAAAGGGACTGCCGAAAAACTAAGCGTGTACACAGAAAAGGGCGAAGTAAAGCAGGGAGA
TGTTTTAAAGCAGCTGTTCCAGGGCACAGACAGGTCGTTGTACAAAGCCATATATTCGTTCGACGTATTCGGGCTGCAGG
AAATTCACGCCTTTAATCGGGACAAGATAGGGGAATTTTTGCTCTTTTCCAGCTTGTTTGGTGCTGAAGCCGTATCAAAG
CTTGATTCAAGGCTGACGAAGGAAAGCGAAAGGCTCTATAAACCAAATGGGCGAAACCCTCAATTGAATCAGGAGCTGGA
AACGCTTAAGCAGCTGGCAGTTAAGCTAAAACAGGCTGAAGCAGAGGAAGCGGGCTATCATCAGCTTCTCGAGGAAAAAC
GAACGCTTGAAGCCCGCCTTGCAGCCGCGGAAACCGAATTGAAAGAGACGGCGGGACATATCCGAATGATTGAGGGAGCG
ATTGAGCGAAAGCCGCTGCTAAATGAAAAAGCGACACTCGAACAGGTGATTGCAGAATTTCCGGAACACGCAGGACAATT
TCCTGCTGACGGACTGCATCAGCTGGAAAAATATGAATCCCATCTTCACCCGAAATCAGCACAGCTTGAGGCGCTTCGGG
TGAAGATGGCGGAGTTGGACAAACAGAGGCAAAAGCTTATCCCCGATAAAGAACTATTGGCGAAAGAAACGCTGATACAA
GAGCTTTCGGCAGCGTTTCATATGTACCAATCCTGGGGAGAACAGCTGGCAGCCATTCAGGCACAGCTGCGCCAGACGTC
TGCGCAGACAGCGGCAGGGCTGGAACAGCTCAATAAAACGGATGAAAATGAGCTCCTGAATATGAACACCTCATATGATT
ATGAATGGCAGCTTCAGCAAGCGGTGCAGCAATATGTACAGGCAAGAGACAGAAAACGGCAGCTTGACGAGACGTTTGAA
CTTGCCCGGCAAGAGCTGGAAGACGCGGAAAAGGCCGTTCGTGCCGCTTCGTCCGCGATTCTTGAAAACAGCCAAAGAAA
AGATAAAGAAGCAGCTCTTAGAGCATATGATGAAACACAGGGGCAGCACCAAGAGCAAGCGAAGCTCAGAGAACAGCTAA
CGTTTTTTGAAAGACAGCAGGCTAAACAGAAAAAGACTGTCATTGCAGCTGGTATGTTGTTCATCGTTCTCTTTTCTCTG
CTGCAGCAATGGATTCCCGCCATAAGTTTCGGCGCTGCACTTATTGTGTATTGGCTTGTTTCAGGAAAGTCGTCTTCTCG
AAACAGCCGAGAGACAAGACAGCCCATGACGGATATTTCACCAGCAGAAGCTGAAATGCTTAGAGAAGCGTTATGGGAGG
ATGACCGCAATAAGCAGCACCTTCTCACACAGCGTGCAGCGCTCCAGCAAAAAGAGGCGGCGTATGAAAGGGTCATCCAG
CAGTTTGAACAGTGGGAAGCCGAAATGGCGCCGTCCTTTACACAGGTTGAGCGTTTTATGAATGAGCTCGGATTTAAGGA
AGATCCATCATTTTTGCTTGATGCATACAGCTTAATGAAGGATGTTAAAAAAGAAGTGAAGAAAAAGCATGAGCTGACCA
TAGAAGCAGGCAGGCTGAAAAAGCACCGGAGAACATTCGAAGAACGCGTCAGTATGCTTCTTCCCGTGAATCAGAGTCAG
GATATCTCCATTTCGGACGCCCTTCACACATTGCGGAAAAACATTGAACGTGAAAAAGAAATTGAGAAGCAAAAAAAGGA
AATTGAAACAGATATTCATTATACAAAGGAACAGATGCTTGAACTTGAGCAAGAAATACAGTATTTCCACGCACAGATTG
AACAGTTATTTGCCGCGGCTGCTGCAAAAGACAGAGATGCGTTTTTTGCCATAGCCGCCATCAGCAGGCAATTGAAGGAT
ACGGAGAACAAGCTTCATCACGTAAACGCACAGCTTCAAGGCGGGTACCCGGAGGAACTTGAACTGGCGGATTCCAATAC
GCTTTCTGAATTGAAAGATAAGCAATTTGTTGAGAATGAAAGAAAAGAGCGGCTGACTGAGGAGATCGAGCAGCTCCGCA
GCCAAATCGCACTGCTCTCAGTCAAACAGGAACAATTAGAGGCTTCGGGAATGGTCTCAGACCTCAAGCTTCAAACCGAA
ATGCAAAAAGAACGTGTGAAAGAAACGGCTAAAAAATGGGCCTCCATACAAATGGTCAAACAGGTGATCAGAAACAAATT
GGAGCGGCATAAAAAAATCGAGCTTCCGCGCCTTCTGGAGACGGCAGGAGAATTTTTCCGTCCTCTGACTGACGGAAACT
ACCAAACCATTTATTTTTCCGAAACGGATGATTCGATCATGGTTATGCATCGCGATGGCACTGTCTATCATGCGGAAGAG
CTGTCACAAGGGACGTGTGAACAGCTGTATACAGCGATCCGCTTTGCTTTGGCCGTTACGCGCCAGGGTGAGTCAAAGCT
TCCGTTCCAGCTTGACGACAGTTTTGTTCATTTTGATCAGGAAAGGCTGAAACGTGTATTACATGTTCTGTATGATTTAT
CTGAAGGCGGTAGGCAGATTTTGTATTTTACATGCCATGAGCACGTAAAAGATGCATTTCATAGCAGCCAAATCATCCAT
TTGGTGTCATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_27)

(5-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Mahalakshmi Krishnamurthy et al. (2010) A novel SMC-like protein, SbcE (YhaN), is involved in DNA double-strand break repair and competence in Bacillus subtilis. Nucleic Acids Research 38(2):455-66. [PMID: 19906728]