Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LUS72_RS21825 Genome accession   NZ_CP089518
Coordinates   4197634..4199955 (-) Length   773 a.a.
NCBI ID   WP_097830589.1    Uniprot ID   A0A2A8U0A8
Organism   Bacillus cereus strain PT1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4192634..4204955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUS72_RS21800 - 4193400..4193576 (-) 177 WP_001242605.1 hypothetical protein -
  LUS72_RS21805 - 4193660..4193935 (-) 276 WP_264449076.1 YrdB family protein -
  LUS72_RS21810 - 4194205..4194645 (-) 441 WP_001266956.1 D-tyrosyl-tRNA(Tyr) deacylase -
  LUS72_RS21815 relA 4194663..4196846 (-) 2184 WP_071747440.1 GTP diphosphokinase -
  LUS72_RS21820 - 4197056..4197568 (-) 513 WP_000346217.1 adenine phosphoribosyltransferase -
  LUS72_RS21825 recJ 4197634..4199955 (-) 2322 WP_097830589.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LUS72_RS21830 - 4200057..4200950 (-) 894 WP_097830588.1 cation diffusion facilitator family transporter -
  LUS72_RS21835 secDF 4201258..4203522 (-) 2265 WP_097830587.1 protein translocase subunit SecDF -
  LUS72_RS21840 - 4203804..4204088 (-) 285 WP_097830586.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 773 a.a.        Molecular weight: 87488.92 Da        Isoelectric Point: 4.8914

>NTDB_id=637895 LUS72_RS21825 WP_097830589.1 4197634..4199955(-) (recJ) [Bacillus cereus strain PT1]
MLQPKTRWKEKEYSEERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKDL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VQRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADVILTEEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDADLESIRAIGSDGSHLKMALRDGQAILDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKGKITMVYFSEEVLNKFSLEDYKEHLVHASEVTQLDDQYIVLLDLPKGTDELRD
LFKMGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLNQKAPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMAEQKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHK

Nucleotide


Download         Length: 2322 bp        

>NTDB_id=637895 LUS72_RS21825 WP_097830589.1 4197634..4199955(-) (recJ) [Bacillus cereus strain PT1]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAGTGAAGAGCGAGTGAGTGAATTAGCAAGTAAATTGCAATT
ATCACCTCTTGTTGTTTCGTTATTCCTCGGTAGAGGATTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCACGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGATTACGATGCGGACGGGGTAAGTAGTACGACTGTCTTATATCTTGCGCTTCAAGAATT
AGGTGCAGAGGTAGAATTTTATATTCCAAACCGTTTTACAGAAGGGTATGGGCCAAACGAAGAAGCGTTTCGTTGGGCGC
ATAGCGCTGGGTTCTCGCTAATTATTACCGTAGATACTGGTATTGCAGCTGTACATGAAGCGAAGGTAGCGAAGGACCTT
GGAATAGATCTTATTATTACAGATCACCATGAGCCACCGCCAGAATTACCAGAAGCACTTGCGATTATTCATCCGAAATT
AGAAGGCGGTGTTTATCCGTTTCACTATTTAGCGGGTGTTGGTGTTGCGTTTAAAGTTGCACATGCACTTTTAGGGCGCG
TTCCAGAACATTTATTGGAAATCGCAGTAATCGGTACAGTAGCTGATTTAGTATCGCTACATGGTGAAAACAGATTGTTA
GTACAGCGCGGGCTTAAACATATGCGTATGACAAGAAATATTGGTTTGAAAGCTTTATTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAAAGTATTGGCTTCTCAATTGCACCGCGTATTAATGCGGTGGGACGTTTAGAAGATGCAACAC
CTGCTGTTCACTTATTATTATCAGACGATCCAGAAGAAGCGAAAGAGCTTGCTGAAGAAATTGATGAGCTGAACAAACTT
CGAAAAGATATTGTAAAGCAAATTACAGAAGAGGCTATCGCTGAAGTGGAAAATAATTTCCCGCCAGAAGAAAACAAAGT
GTTAGTGCTTGCAAAAGAAGGCTGGAATCCAGGTGTAATTGGGATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTGAGCATTGACCCTATAAAAGAAACGGCAAAGGGTTCAGCGCGTAGTATTGCAGGGTTTGATTTATTT
GCTAACTTGTCTGATTGTAGAGAGTTATTGCCACACTTCGGGGGACACCCGATGGCAGCCGGAATGACGCTACATATGAA
TGATGTAGATGAATTAAGACGTAGGTTAAATGAGCAAGCTGATGTGATTTTAACTGAGGAAGATTTTATTCCAATTACAG
CAGTTGATGCTTTTTGTAAGGTGGAGGATGTAACTCTTGCTGCAATTGAGGATATGCAAAAGTTAGCGCCGTTTGGTGTT
GGAAATCCAAAACCACGTATTGCGGTAAAAGATGCTGATTTGGAAAGTATTCGTGCAATCGGATCAGATGGTTCACATTT
GAAAATGGCACTTCGTGACGGACAGGCAATACTGGATACAATTGGATTCGGATTTGGTGCGTATGCGAAAGAAATTTCTC
CAGTTGCGAAAGTTTCTGTAATAGGAGAGGCATCCATTAATGAATGGAATAATTTTAAGAAGCCACAATTAATGGTACAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGTAGTATGCGTAATGTGGAAGCAAATTTAGCAGAACTTCCGAA
AGGAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAGTTTTCTTTAGAAGACTATAAAGAACATCTCGTGC
ATGCATCAGAGGTCACACAGTTAGATGATCAATACATCGTTTTGCTTGATTTGCCGAAAGGAACAGATGAATTACGTGAC
CTATTTAAAATGGGATTCCCATCGCGAATTTATACGTTATTTTATCAAGAGAATAACCATTTATTTAGTACAGTCCCAAC
GAGGGAACATTTTAAATGGTACTACTCTTTCTTGAACCAAAAAGCACCATTTTCTTTAAGACAATATGGGGAACAACTAT
GTCGTCATAAAGGTTGGTCGAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCCGAACAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAT
AA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHHA1 domain (DHHA1)

(315-439)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A8U0A8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.584

100

0.527