Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PALA12_RS00370 Genome accession   NZ_CP106681
Coordinates   82186..82611 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PALA12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 77186..87611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA12_RS00350 (PALA12_00070) ileS 77343..80174 (+) 2832 WP_003102617.1 isoleucine--tRNA ligase -
  PALA12_RS00355 (PALA12_00071) lspA 80167..80676 (+) 510 WP_003094728.1 signal peptidase II -
  PALA12_RS00360 (PALA12_00072) fkpB 80669..81109 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PALA12_RS00365 (PALA12_00073) ispH 81195..82139 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PALA12_RS00370 (PALA12_00074) comF 82186..82611 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PALA12_RS00375 (PALA12_00075) pilY2 82608..82955 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  PALA12_RS00380 (PALA12_00076) pilY1 82957..86448 (-) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  PALA12_RS00385 (PALA12_00077) pilX 86460..87047 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=635677 PALA12_RS00370 WP_003094721.1 82186..82611(-) (comF) [Pseudomonas aeruginosa strain PALA12]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=635677 PALA12_RS00370 WP_003094721.1 82186..82611(-) (comF) [Pseudomonas aeruginosa strain PALA12]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAATCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383