Detailed information NTG0063552

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PA1R_RS20050
• Genome accession: NC_022806
• Coordinates: 4354288..4355436 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa PA1R
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4349288..4360436

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA1R_RS20025	-	4350439..4350735 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PA1R_RS20030 (PA1R_gp3903)	-	4350732..4351325 (-)	594	WP_003084544.1	YggT family protein	-
PA1R_RS20035 (PA1R_gp3904)	proC	4351336..4352157 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PA1R_RS20040 (PA1R_gp3905)	-	4352169..4352861 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PA1R_RS20045 (PA1R_gp3906)	pilT	4353076..4354110 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PA1R_RS20050 (PA1R_gp3907)	pilU	4354288..4355436 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PA1R_RS20055 (PA1R_gp3908)	czcD	4355444..4356343 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PA1R_RS20060 (PA1R_gp3909)	-	4356497..4356901 (+)	405	WP_003084560.1	NINE protein	-
PA1R_RS20065 (PA1R_gp3910)	-	4357144..4358517 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PA1R_RS20070 (PA1R_gp3911)	-	4358514..4359698 (+)	1185	WP_023518288.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=63552 PA1R_RS20050 WP_003100959.1 4354288..4355436(+) (pilU) [Pseudomonas aeruginosa PA1R]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=63552 PA1R_RS20050 WP_003100959.1 4354288..4355436(+) (pilU) [Pseudomonas aeruginosa PA1R]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382