Detailed information NTG0635078

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LSP16_RS02635
• Genome accession: NZ_CP089067
• Coordinates: 529701..530849 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF19
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 524701..535849

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSP16_RS02615 (LSP16_02565)	-	525439..526623 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
LSP16_RS02620 (LSP16_02570)	-	526620..527993 (-)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
LSP16_RS02625 (LSP16_02575)	-	528236..528640 (-)	405	WP_003084560.1	NINE protein	-
LSP16_RS02630 (LSP16_02580)	czcD	528794..529693 (+)	900	WP_219832742.1	cation diffusion facilitator family transporter	-
LSP16_RS02635 (LSP16_02585)	pilU	529701..530849 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LSP16_RS02640 (LSP16_02590)	pilT	531027..532061 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LSP16_RS02645 (LSP16_02595)	-	532276..532968 (+)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
LSP16_RS02650 (LSP16_02600)	proC	532980..533801 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LSP16_RS02655 (LSP16_02605)	-	533812..534405 (+)	594	WP_003084544.1	YggT family protein	-
LSP16_RS02660 (LSP16_02610)	-	534402..534698 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=635078 LSP16_RS02635 WP_003100959.1 529701..530849(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF19]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=635078 LSP16_RS02635 WP_003100959.1 529701..530849(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF19]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382