Detailed information NTG0063205

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: SAGCMC97051_RS04060
• Genome accession: NZ_AP020310
• Coordinates: 709614..710399 (+)
• Length: 261 a.a.
• NCBI ID: WP_000825431.1
• Uniprot ID: Q8E0P2
• Organism: Streptococcus agalactiae strain GCMC97051
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 704614..715399

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAGCMC97051_RS04030 (SAGCMC97051_07160)	-	705731..706174 (+)	444	WP_001048166.1	hypothetical protein	-
SAGCMC97051_RS04035 (SAGCMC97051_07170)	-	706259..706525 (+)	267	WP_001865676.1	hypothetical protein	-
SAGCMC97051_RS04040	-	706626..706754 (+)	129	WP_000962057.1	hypothetical protein	-
SAGCMC97051_RS04045 (SAGCMC97051_07180)	-	706763..707083 (+)	321	WP_001865677.1	hypothetical protein	-
SAGCMC97051_RS04050 (SAGCMC97051_07190)	-	707180..707851 (+)	672	WP_000891268.1	ATP-binding cassette domain-containing protein	-
SAGCMC97051_RS04055 (SAGCMC97051_07200)	-	707908..709353 (-)	1446	WP_000750933.1	FAD/NAD(P)-binding protein	-
SAGCMC97051_RS04060 (SAGCMC97051_07210)	endA	709614..710399 (+)	786	WP_000825431.1	DNA/RNA non-specific endonuclease	Machinery gene
SAGCMC97051_RS04065 (SAGCMC97051_07220)	-	710477..711115 (-)	639	WP_000478529.1	VTT domain-containing protein	-
SAGCMC97051_RS04070 (SAGCMC97051_07230)	-	711333..711989 (+)	657	WP_000121094.1	ABC transporter ATP-binding protein	-
SAGCMC97051_RS04075 (SAGCMC97051_07240)	-	711986..712759 (+)	774	WP_000012390.1	ABC transporter permease	-
SAGCMC97051_RS04080 (SAGCMC97051_07250)	-	712923..713741 (+)	819	WP_000601007.1	hypothetical protein	-
SAGCMC97051_RS04085 (SAGCMC97051_07260)	-	713778..714662 (-)	885	WP_000354840.1	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 261 a.a.
• Molecular weight: 29424.86 Da
• Isoelectric Point: 8.9989

>NTDB_id=63205 SAGCMC97051_RS04060 WP_000825431.1 709614..710399(+) (endA) [Streptococcus agalactiae strain GCMC97051]
MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSVDTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLN
ADVNVKAYVQNTIDNQQRLSTANAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDTDLVPFAMHLEAKSQDGTLEFNV
AIPNTQASYTMDYATGEITLN

Nucleotide

• Length: 786 bp

>NTDB_id=63205 SAGCMC97051_RS04060 WP_000825431.1 709614..710399(+) (endA) [Streptococcus agalactiae strain GCMC97051]
ATGAAAAGATTACATAAACTGTTTATAACCGTAATTGCTACATTAGGTATGTTGGGGGTAATGACCTTTGGTCTTCCAAC
GCAGCCGCAAAACGTAACGCCGATAGTACATGCTGATGTCAATTCATCTGTTGATACGAGCCAGGAATTTCAAAATAATT
TAAAAAATGCTATTGGTAACCTACCATTTCAATATGTTAATGGTATTTATGAATTAAATAATAATCAGACAAATTTAAAT
GCTGATGTCAATGTTAAAGCGTATGTTCAAAATACAATTGACAATCAACAAAGACTATCAACTGCTAATGCAATGCTTGA
TAGAACCATTCGTCAATATCAAAATCGCAGAGATACCACTCTTCCCGATGCAAATTGGAAACCATTAGGTTGGCATCAAG
TAGCTACTAATGACCATTATGGACATGCAGTCGACAAGGGGCATTTAATTGCCTATGCTTTAGCTGGAAATTTCAAAGGT
TGGGATGCTTCCGTGTCAAATCCTCAAAATGTTGTCACACAAACAGCTCATTCCAACCAATCAAATCAAAAAATCAATCG
TGGACAAAATTATTATGAAAGCTTAGTTCGTAAGGCGGTTGACCAAAACAAACGTGTTCGTTACCGTGTAACTCCATTGT
ACCGTAATGATACTGATTTAGTTCCATTTGCAATGCACCTAGAAGCTAAATCACAAGATGGCACATTAGAATTTAATGTT
GCTATTCCAAACACACAAGCATCATACACTATGGATTATGCAACAGGAGAAATAACACTAAATTAA

Domains

Predicted by InterProScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (77-235)

3D structure

• AlphaFold DB: Q8E0P2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	51.515	75.862	0.391
endA	Streptococcus pneumoniae D39	51.515	75.862	0.391
endA	Streptococcus pneumoniae R6	51.515	75.862	0.391
endA	Streptococcus pneumoniae TIGR4	51.515	75.862	0.391