Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   LMD38_RS20745 Genome accession   NZ_CP086328
Coordinates   3991526..3995041 (-) Length   1171 a.a.
NCBI ID   WP_000058555.1    Uniprot ID   A0AAP5L6Q6
Organism   Bacillus pacificus strain anQ-h4     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3986526..4000041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMD38_RS20720 (LMD38_20720) gerPD 3986569..3986763 (+) 195 WP_001102341.1 spore germination protein GerPD -
  LMD38_RS20725 (LMD38_20725) - 3986779..3987165 (+) 387 WP_000902338.1 spore germination protein GerPE -
  LMD38_RS20730 (LMD38_20730) gerPF 3987208..3987423 (+) 216 WP_001141566.1 spore germination protein GerPF -
  LMD38_RS20735 (LMD38_20735) - 3987504..3987791 (-) 288 WP_016716842.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  LMD38_RS20740 (LMD38_20740) addA 3987804..3991529 (-) 3726 WP_062822692.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LMD38_RS20745 (LMD38_20745) addB 3991526..3995041 (-) 3516 WP_000058555.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LMD38_RS20750 (LMD38_20750) lepB 3995158..3995721 (-) 564 WP_061183253.1 signal peptidase I -
  LMD38_RS20755 (LMD38_20755) - 3995778..3996371 (-) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  LMD38_RS20760 (LMD38_20760) - 3996725..3997321 (-) 597 WP_029141079.1 DUF4306 domain-containing protein -
  LMD38_RS20765 (LMD38_20765) - 3997561..3998142 (-) 582 WP_061183254.1 competence protein ComK -
  LMD38_RS20770 (LMD38_20770) - 3998426..3998611 (+) 186 WP_000391455.1 hypothetical protein -
  LMD38_RS20775 (LMD38_20775) - 3998934..3999155 (+) 222 WP_000412680.1 hypothetical protein -
  LMD38_RS20780 (LMD38_20780) cspA 3999493..3999696 (-) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134159.23 Da        Isoelectric Point: 5.2121

>NTDB_id=624321 LMD38_RS20745 WP_000058555.1 3991526..3995041(-) (addB) [Bacillus pacificus strain anQ-h4]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTLDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTIHTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=624321 LMD38_RS20745 WP_000058555.1 3991526..3995041(-) (addB) [Bacillus pacificus strain anQ-h4]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGCCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAAGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTATTCCAAAAAGCAGCGGAGCAAAATGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGCGCCGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAGTACTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGCTTCCATTCGT
TTTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTGACAATCACGTTAACGCTAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAACCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAGAAATTTAACGGGGAAGCAAGTGTAACGATTCATACAGCCGCGAATTTA
CGAGCGGAAGTAGAAGGTGTTGCTCGTGAAATTCGTAGACTTGTGGCAGATGAAGACTATCGTTACCGAGATATTGCAGT
TCTTCTTCGTAACGGAGAAAGTTATTACGATGTAATGCGGACTCTATTTACAGATTATAATATCCCGCACTTTATCGATG
AAAAACGCCCGATGTCACATCATCCGTTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTATTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCATACGGTGTACAAGGGAAAAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGAACGGGATGATTACAGACAGCGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCCGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACGGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTTGACGTTCCGAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTTGTTGAGATGCTTGGCGAAGAAAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTCGTCATTGGAGTGAATGAAGGTG
TGATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAAGAAAGAGATGTTCTTAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTTGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
CATTTCATGCCCGCTTGCAGATGAGGAAGGAAAGACGTTACTTGCTTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAACGACGTAAATGATTTATCACGTTCGGAACAAATTTCATATGTAGCAACACCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGCAACTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATATGGAGACAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTTGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTCAAACTGGATGC
GCCAGATATTGGGGAACTATTCCATGCAGCACTGAAGAGAATTGCGGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGCTGTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAGCA
CGCGAAATCGAGTGGTTTCGTACCAGTTGATTTAGAAGTACCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCAATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGA
ACATTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCGCATACGTGGATGAAAAAAGGCGGCACGGCATCACCAGCTGGTG
TATTGTATTTCCACATTCATAACCCAATTGTGGAGGTAAAAGGTGACGCATCTGAAGCAGAAATTGAGAAAGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAACAAGAGTTTA
ACGTCCTCCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACGGAAGGCGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCGGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAATTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.232

100

0.493