Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   SIR_RS12265 Genome accession   NC_022246
Coordinates   489746..490591 (+) Length   281 a.a.
NCBI ID   WP_021002539.1    Uniprot ID   A0AAE8G4Y2
Organism   Streptococcus intermedius B196     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 484746..495591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIR_RS12245 (SIR_0493) - 485344..486969 (+) 1626 WP_021002537.1 SpaA isopeptide-forming pilin-related protein -
  SIR_RS12250 (SIR_0494) - 487024..487860 (+) 837 WP_041787387.1 class C sortase -
  SIR_RS19585 (SIR_0495) - 487966..488196 (+) 231 WP_014829739.1 DUF1146 family protein -
  SIR_RS12255 (SIR_0496) murA 488234..489514 (+) 1281 WP_003072160.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  SIR_RS12260 - 489507..489683 (+) 177 WP_003072163.1 DNA-directed RNA polymerase subunit beta -
  SIR_RS12265 (SIR_0497) endA 489746..490591 (+) 846 WP_021002539.1 DNA/RNA non-specific endonuclease Machinery gene
  SIR_RS12270 (SIR_0498) - 490745..492481 (+) 1737 WP_021002540.1 ABC transporter ATP-binding protein -
  SIR_RS12275 (SIR_0499) - 492491..494260 (+) 1770 WP_021002541.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 281 a.a.        Molecular weight: 30714.35 Da        Isoelectric Point: 10.3160

>NTDB_id=61872 SIR_RS12265 WP_021002539.1 489746..490591(+) (endA) [Streptococcus intermedius B196]
MSQRKSNQKVMQSVIGGIIALSLAIGAYYFRANNNGTVHQDSSAINKSLEQHHAGPSKDLAQSVLTGNVRSQFKGKIEWN
GAGAFIVNENKNDLDAKVGSIPYADNKTKLVRGRTVPTVANALLSKATRQYRNREETGNGSTTWTPAGWHQVKHLSGEYH
HAVDRGHLLGYALIGNLKGFDASTSNPKNIAVQTAWANQANASHSTGQNFYETKVRKALDKNKRVRYRVTLIYASEQDLV
PVGSHIEAKSSDGSLEINVFVPNVQTGIRLNYQTGEVTVTN

Nucleotide


Download         Length: 846 bp        

>NTDB_id=61872 SIR_RS12265 WP_021002539.1 489746..490591(+) (endA) [Streptococcus intermedius B196]
ATGTCGCAGAGAAAATCCAATCAAAAAGTAATGCAGAGTGTTATTGGAGGGATTATTGCACTTAGTTTAGCTATTGGTGC
TTATTATTTTCGAGCAAATAATAATGGTACAGTTCATCAAGATAGTTCTGCCATTAACAAAAGTCTCGAGCAACATCATG
CGGGTCCTAGTAAAGATCTGGCTCAGAGTGTATTGACAGGTAATGTGCGTTCACAATTTAAAGGGAAAATCGAGTGGAAT
GGCGCAGGAGCTTTTATTGTCAATGAGAATAAAAATGATTTAGATGCTAAAGTGGGAAGTATTCCTTATGCGGATAATAA
GACAAAATTGGTCAGAGGTCGAACTGTTCCGACGGTAGCAAATGCTCTTTTGTCTAAAGCTACTCGTCAGTATAGGAATC
GTGAAGAAACAGGTAACGGTTCAACAACTTGGACACCTGCTGGCTGGCATCAGGTGAAGCATTTATCGGGTGAATATCAT
CATGCAGTTGACCGTGGACATCTGCTAGGCTATGCCTTGATTGGTAACTTGAAAGGCTTTGATGCTTCGACTAGTAATCC
CAAAAATATTGCGGTGCAAACGGCTTGGGCGAATCAAGCAAATGCTAGCCATTCTACAGGTCAAAATTTTTATGAAACGA
AAGTACGTAAAGCTCTTGATAAAAATAAACGAGTTCGGTATCGAGTGACCTTGATTTACGCAAGTGAGCAGGATTTAGTA
CCAGTTGGTTCACATATTGAAGCCAAGTCTAGCGACGGTAGTTTGGAAATAAATGTCTTTGTGCCAAACGTACAGACAGG
AATTCGATTAAATTATCAGACAGGAGAAGTGACGGTCACCAACTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(112-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

68.613

97.509

0.669
  endA Streptococcus pneumoniae D39

68.613

97.509

0.669
  endA Streptococcus pneumoniae R6

68.613

97.509

0.669
  endA Streptococcus pneumoniae TIGR4

68.613

97.509

0.669

Multiple sequence alignment