Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LIS78_RS23470 Genome accession   NZ_CP085442
Coordinates   4513859..4516201 (-) Length   780 a.a.
NCBI ID   WP_252284412.1    Uniprot ID   -
Organism   Priestia megaterium strain KF18     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4508859..4521201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS78_RS23445 (LIS78_23465) - 4509252..4509428 (-) 177 WP_013059305.1 hypothetical protein -
  LIS78_RS23450 (LIS78_23470) - 4509595..4510479 (+) 885 WP_229754521.1 N-acetylmuramoyl-L-alanine amidase -
  LIS78_RS23455 (LIS78_23475) dtd 4510470..4510931 (-) 462 WP_013059307.1 D-aminoacyl-tRNA deacylase -
  LIS78_RS23460 (LIS78_23480) - 4510947..4513145 (-) 2199 WP_116073761.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  LIS78_RS23465 (LIS78_23485) - 4513331..4513843 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  LIS78_RS23470 (LIS78_23490) recJ 4513859..4516201 (-) 2343 WP_252284412.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LIS78_RS23475 (LIS78_23495) secDF 4516342..4518579 (-) 2238 WP_195781809.1 protein translocase subunit SecDF -
  LIS78_RS23480 (LIS78_23500) - 4518717..4519010 (-) 294 WP_028411692.1 post-transcriptional regulator -
  LIS78_RS23485 (LIS78_23505) spoVB 4519075..4520685 (+) 1611 WP_252284413.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87860.45 Da        Isoelectric Point: 4.6980

>NTDB_id=618155 LIS78_RS23470 WP_252284412.1 4513859..4516201(-) (recJ) [Priestia megaterium strain KF18]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDVQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDTLNKE
RQQIVNDITEEAVEFVEGMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASDVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQNEEQAKACMLAGEQIVLLDLPPSQ
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSTKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFERSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=618155 LIS78_RS23470 WP_252284412.1 4513859..4516201(-) (recJ) [Priestia megaterium strain KF18]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
TACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGCACTTCCGTTATGTTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCGGCATTTGATAAAATAA
GTGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCTGTCGAAGAAACTGCGTATGCACGTGAATTA
AATTTTGATTTAATTATTACTGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGTAAAGGTCTTGACTATCTCAAGCTCACGAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAGCG
TGAAGAAATTAATGAAGATACAATTGGATTTGCGATGGCTCCTCGTATTAATGCAGTAGGACGTCTTCAAGATGCAGACC
CAGCTGTTCATTTGCTTATGACCACAAATGCTGAAGAGGCAAAAGGTTTAGCTAAAGAAATTGATACGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAAGAAGCCGTTGAATTTGTTGAAGGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAAGGAGAAGGCTGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCGACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTAGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTCCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTCGTGTTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCGGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCATGTATGCTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTCACAG
AAGTGGCTTGAATGTTTGTTTGTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTGACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTACGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTGAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(305-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.627

100

0.558