Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LIT33_RS23295 Genome accession   NZ_CP085415
Coordinates   4458544..4460886 (-) Length   780 a.a.
NCBI ID   WP_252287747.1    Uniprot ID   -
Organism   Priestia megaterium strain CK7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4453544..4465886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT33_RS23270 (LIT33_23285) - 4453937..4454113 (-) 177 WP_013059305.1 hypothetical protein -
  LIT33_RS23275 (LIT33_23290) - 4454280..4455164 (+) 885 WP_223268964.1 N-acetylmuramoyl-L-alanine amidase -
  LIT33_RS23280 (LIT33_23295) dtd 4455155..4455616 (-) 462 WP_013059307.1 D-aminoacyl-tRNA deacylase -
  LIT33_RS23285 (LIT33_23300) - 4455632..4457830 (-) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  LIT33_RS23290 (LIT33_23305) - 4458016..4458528 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  LIT33_RS23295 (LIT33_23310) recJ 4458544..4460886 (-) 2343 WP_252287747.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LIT33_RS23300 (LIT33_23315) secDF 4461027..4463264 (-) 2238 WP_252287748.1 protein translocase subunit SecDF -
  LIT33_RS23305 (LIT33_23320) - 4463402..4463695 (-) 294 WP_013059312.1 post-transcriptional regulator -
  LIT33_RS23310 (LIT33_23325) spoVB 4463819..4465369 (+) 1551 WP_013059313.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87949.59 Da        Isoelectric Point: 4.7511

>NTDB_id=617986 LIT33_RS23295 WP_252287747.1 4458544..4460886(-) (recJ) [Priestia megaterium strain CK7]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKEAFDKISAGGYQLVITVDTGISAVEETAYVREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVESMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQDEEQAKACMFAGEQIVLLDLPPSR
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=617986 LIT33_RS23295 WP_252287747.1 4458544..4460886(-) (recJ) [Priestia megaterium strain CK7]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGCATTCAGCAGGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCTGTTATGCTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGAGGCATTTGATAAAATAA
GCGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGTACGTGAATTA
AACTTTGATTTAATTATTACTGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTAATTGGGACGATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGATTATCTCAAGCTCACGAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATTGGATTTGCGATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCCGATC
CAGCTGTTCATTTACTTATGACGACAAATGCTGAAGAGGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAAGAAGCCGTTGAATTTGTTGAGAGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAATTCTATAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGTTTTGACTTATTC
GCGAACTTATCTAAGTGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCATGCTTCAACAATTGATAAATATAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTCACGG
AAATGGCTTGAATGTTTGTTTGTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(305-439)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.641

100

0.556