Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LIT28_RS22460 Genome accession   NZ_CP085409
Coordinates   4363550..4365889 (-) Length   779 a.a.
NCBI ID   WP_063222853.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain KF1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4358550..4370889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT28_RS22430 (LIT28_22445) - 4359199..4359375 (-) 177 WP_001242605.1 hypothetical protein -
  LIT28_RS22435 (LIT28_22450) - 4359461..4359652 (-) 192 WP_063222857.1 hypothetical protein -
  LIT28_RS22440 (LIT28_22455) - 4359737..4359967 (-) 231 Protein_4394 YrdB family protein -
  LIT28_RS22445 (LIT28_22460) - 4360139..4360579 (-) 441 WP_001266956.1 D-tyrosyl-tRNA(Tyr) deacylase -
  LIT28_RS22450 (LIT28_22465) relA 4360597..4362780 (-) 2184 WP_063222854.1 GTP diphosphokinase -
  LIT28_RS22455 (LIT28_22470) - 4362990..4363502 (-) 513 WP_000346213.1 adenine phosphoribosyltransferase -
  LIT28_RS22460 (LIT28_22475) recJ 4363550..4365889 (-) 2340 WP_063222853.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LIT28_RS22465 (LIT28_22480) - 4365991..4366884 (-) 894 WP_063222852.1 cation diffusion facilitator family transporter -
  LIT28_RS22470 (LIT28_22485) secDF 4367038..4369302 (-) 2265 WP_242256430.1 protein translocase subunit SecDF -
  LIT28_RS22475 (LIT28_22490) - 4369573..4369857 (-) 285 WP_000884549.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88062.56 Da        Isoelectric Point: 4.9330

>NTDB_id=617882 LIT28_RS22460 WP_063222853.1 4363550..4365889(-) (recJ) [Bacillus thuringiensis strain KF1]
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GVDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDAAPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVETNFTPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVDAWQLFDWRSMRNVESNLAELPKEKITMVYFSEEVLHKFSLEDYKEHIMHASEVTELNDQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKAPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=617882 LIT28_RS22460 WP_063222853.1 4363550..4365889(-) (recJ) [Bacillus thuringiensis strain KF1]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGGCGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACTGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTGGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCCTTGCAAGAATT
AGGTGCAGATGTAGAATTTTATATTCCAAACCGTTTTACAGAAGGTTATGGACCAAACGAAGAAGCATTTCGTTGGGCGC
ATAGCGCAGGATTCTCGCTAATTATTACTGTCGATACTGGTATCGCAGCAGTACATGAAGCGAAGGTAGCGAAGGAGCTT
GGAGTAGATCTTATTATTACAGATCACCATGAGCCACCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTGTATCCATTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCGGTAATTGGTACGGTAGCCGATTTAGTATCACTTCATGGTGAGAATAGATTGCTA
GTGAAACGCGGCTTAAAACATATGCGTATGACGAAAAATATTGGACTAAAGGCGCTATTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCGCGTATTAATGCAGTTGGCCGTTTAGAAGATGCAGCGC
CTGCTGTACATCTTTTATTATCAGATGATCCAGAAGAAGCGAAAGAGCTGGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTGGAAACTAATTTCACGCCTGAAGAAAATAAAGT
ATTAGTGCTTGCAAAAGAAGGCTGGAATCCAGGTGTAATTGGAATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTAAGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCACGTAGTATTGCAGGATTCGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGCTATTGCCACACTTCGGAGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGTCGCTTAAATGAGCAAGCAGAGGTAATTTTAACAGAAGAAGATTTTATTCCGATTACAG
CTGTTGATGCTTTTTGTAAAGTGGAGGACGTAACACTTGCAGCGATTGAAGATATGCAAAAGTTGGCGCCATTTGGTGTT
GGAAATCCGAAACCACGTATTGCAGTGAAAGATGCAGAGCTAGAAAGTATTCGTGCAATCGGATCAGACGGTTCTCATTT
GAAAATGGCTCTTCGCGATGGACAGGCAACACTGGATACAATCGGATTTGGTTTTGGCGCGTATGCGAAAGAAATTTCTC
CAGTTGCAAAGGTATCTGTAATAGGGGAAGCGTCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTACAA
GATATTGCTGTAGACGCATGGCAATTATTTGATTGGCGTAGTATGCGTAACGTAGAATCAAATTTAGCAGAACTTCCAAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGCATATTATGC
ATGCATCAGAAGTAACTGAGTTAAATGATCAATACATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGAGAT
CTATTTAAAGTTGGGTTCCCGTCGCGAATTTATACACTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGAGAACACTTTAAATGGTACTATTCGTTCTTGAGCCAAAAAGCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-439)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.455

99.358

0.521