Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LIS79_RS12735 Genome accession   NZ_CP085389
Coordinates   2446585..2448918 (-) Length   777 a.a.
NCBI ID   WP_060832475.1    Uniprot ID   -
Organism   Bacillus altitudinis strain CMF5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2441585..2453918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS79_RS12720 (LIS79_12725) dtd 2443234..2443677 (-) 444 WP_008342725.1 D-aminoacyl-tRNA deacylase -
  LIS79_RS12725 (LIS79_12730) - 2443696..2445906 (-) 2211 WP_008342726.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  LIS79_RS12730 (LIS79_12735) - 2446049..2446561 (-) 513 WP_008342729.1 adenine phosphoribosyltransferase -
  LIS79_RS12735 (LIS79_12740) recJ 2446585..2448918 (-) 2334 WP_060832475.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LIS79_RS12740 (LIS79_12745) - 2449000..2449356 (-) 357 WP_019743029.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  LIS79_RS12745 (LIS79_12750) - 2449456..2449944 (-) 489 WP_046344113.1 cation:proton antiporter regulatory subunit -
  LIS79_RS12750 (LIS79_12755) secDF 2450145..2452373 (-) 2229 WP_017367708.1 protein translocase subunit SecDF -
  LIS79_RS12755 (LIS79_12760) - 2452415..2452711 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 777 a.a.        Molecular weight: 87648.39 Da        Isoelectric Point: 4.8057

>NTDB_id=617357 LIS79_RS12735 WP_060832475.1 2446585..2448918(-) (recJ) [Bacillus altitudinis strain CMF5]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVQRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVELLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSRRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDMDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGADQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLQADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDEMTVQAIEEVGLLSPFGMQN
PKPFIMIEDAQLEDIRTIGANQNHIKMSLKDEDKLLDCVGFHQGKLKEELVPGSQISVVGEVSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDTAKTMVFCFDEETKRQSELVDIPIHLIDAENVSTFDVQEKYVIFADVPADEHLLKL
LLENQQPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIE
NGVLSIVRDAPKKDLTASSSYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2334 bp        

>NTDB_id=617357 LIS79_RS12735 WP_060832475.1 2446585..2448918(-) (recJ) [Bacillus altitudinis strain CMF5]
ATGTTATTATCCAAAATGCGTTGGGAATATGAACATCCTAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCACTTCTTGTACAAAGAGGGCTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCTTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATTGAAGAGCAG
GAATCGATTGTGATCTTCGGAGATTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACACTAAAAGAACG
ATCTGCTAAGGTAGACTTTTACATTCCAGATCGATTTAAAGAAGGATATGGGCCAAACGAACAGGCTTTCCGATATATCA
AAGAGCAAGGGGCATCGCTTGTTATCACCGTAGATACAGGGATCGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTGATCATCACAGATCACCATGAACCAAGCGAGGAACTGCCTGAGGCACTTGCGATTGTACATCCTAAGCA
GCCTGGGTGTGAGTATCCGTTTAAAGAGCTTGCGGGTGTCGGTGTTGCGTTTAAAGTGGCACATGCACTCCTTGGTAAAC
TCCCAGTAGAATTATTAGATCTTGCTGCAATCGGTACAATTGCGGATCTTGTCCCGCTGCATGATGAAAATAGATGGCTT
GCAAAAAAAGGCCTGATGCAGCTTCGTCAATCTAGACGGCCTGGGTTAAAAGCGTTATTAAAAGAAGCAGGCGCCACTCT
TGAAGAAGCAAATGAGGAAACAGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGACGAATTGAGCAGGCAGATC
CGGCTGTTCATCTTCTCATGACTGAGGATATGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAATTGAACAAAGAA
CGGCAGAAAATTGTGAGTACGATCACGGAAGAAGCCATTCAAATGGTCGAAGAGACAGGTGCTGACCAGTCTGCTATCGT
CGTAGCAAAAGCTGGCTGGAATCCAGGCGTTGTCGGCATTGTTGCCTCAAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGACGAAGAAACGCAGATGGCGAAAGGGTCAGCTCGAAGTATTCCTGGCTTTGATTTATTTTTCCAT
TTGAGCAAGTGCCGTGACATCCTGCCGCATTTCGGTGGACACCCGATGGCAGCAGGAATGACACTTCAAGCGGATGATGT
TCCTTTACTTAGGGAAAGATTGAATCAATATGCAAATGAAACATTGACAGAAGAAGATTTCATTCCAATTCAACGGGTCG
ATGCAGTATGTAAAGTGGACGAAATGACGGTGCAAGCCATTGAAGAAGTGGGACTGCTGTCCCCGTTTGGCATGCAGAAT
CCTAAGCCGTTCATTATGATTGAAGACGCCCAGCTTGAAGACATCCGAACGATCGGGGCCAATCAAAATCACATTAAAAT
GTCACTAAAAGACGAAGACAAGCTGCTCGACTGTGTCGGTTTTCACCAAGGCAAGCTAAAAGAAGAACTTGTCCCAGGGA
GTCAAATTTCTGTCGTTGGTGAGGTCTCTATTAATGAATGGAATAACCGAAAGAAACCTCAGCTCATGCTAAAAGACGCC
AGAGTCGATGAATGGCAGCTCTTTGATTTACGCGGGAAAAAAGATTGGGAAAAGAAAGTATCAACACTTGATACAGCAAA
AACGATGGTCTTCTGTTTTGACGAAGAAACAAAAAGGCAATCGGAGCTGGTGGATATTCCGATTCACCTTATTGATGCAG
AAAATGTATCCACGTTTGATGTGCAGGAAAAGTATGTGATCTTTGCCGATGTTCCTGCAGATGAACACTTGCTCAAGCTG
CTTTTAGAAAATCAACAACCAGCAAGAATTTATACGGTGTTTCAGCGGAAAGAAGATCACTTTATGTCCTCATTCCCGAG
CCGTGATCAATTCAAATGGTTCTATGGGTTCTTGTTCAAACGGGGAAGTTTTCCTATTAAAGAACAAGGCATGGAGCTTG
CCAAACATAGAGGTTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAG
AATGGTGTGCTGTCAATAGTGCGTGATGCCCCTAAAAAAGATTTAACCGCATCATCGTCTTATACAGCAAAACAACGATT
GATGGAACTCGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCT
CACCTGTGCTTTGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-766)

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(314-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.181

100

0.672