Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LG298_RS20695 Genome accession   NZ_CP085255
Coordinates   4081274..4083643 (-) Length   789 a.a.
NCBI ID   WP_250800986.1    Uniprot ID   -
Organism   Cytobacillus firmus strain ABH-509     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4076274..4088643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG298_RS20680 (LG298_20530) - 4076276..4078471 (-) 2196 WP_061791146.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  LG298_RS20685 (LG298_20535) ltrA 4078913..4080172 (-) 1260 WP_404322164.1 group II intron reverse transcriptase/maturase -
  LG298_RS20690 (LG298_20540) - 4080771..4081283 (-) 513 WP_048010354.1 adenine phosphoribosyltransferase -
  LG298_RS20695 (LG298_20545) recJ 4081274..4083643 (-) 2370 WP_250800986.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LG298_RS20700 (LG298_20550) - 4083803..4084120 (-) 318 WP_048010356.1 lipopolysaccharide assembly LapA domain-containing protein -
  LG298_RS20705 (LG298_20555) - 4084145..4085047 (-) 903 WP_222497680.1 cation diffusion facilitator family transporter -
  LG298_RS20710 (LG298_20560) secDF 4085182..4087446 (-) 2265 WP_197213322.1 protein translocase subunit SecDF -
  LG298_RS20715 (LG298_20565) - 4087735..4088031 (-) 297 WP_222497678.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 789 a.a.        Molecular weight: 88026.38 Da        Isoelectric Point: 5.0960

>NTDB_id=616590 LG298_RS20695 WP_250800986.1 4081274..4083643(-) (recJ) [Cytobacillus firmus strain ABH-509]
MLHSRTRWIVRQTEEDKVQRLSKELNISPLAASLLINRGMDTVDSARYFLMDNEQEFHDPFLMTDMAIAVSRIKEAIEKQ
EPILIFGDYDADGVTSTSVMVMALKDLGAAVQFYIPNRFTEGYGPNIPAFEHAAEIGIRLIITVDTGISAVNEAKAAKEL
GMDLIITDHHEPGPELPEALAIVHPKHPQSRYPFRELAGVGVAFKLAHALYGEMPEHLLEIAAIGTIADLVSLTGENRLI
AKRGIQKLKSTKNTGLNALFKAARIELPSISEETIGFTIGPRLNAAGRLESADPAVDLLLTNDPYEAQAIAEEIEMLNKE
RQSIVNDIAAQAVHEVETNFPISENSVIVVGKEGWNAGVIGIVASKLVEKFYRPAIVLSYDSEKGLAKGSARSIEGFDLF
KNLSECRDILPHFGGHPMAAGMTLSIDSVDDLRSRLNTLANEQLSEEDFIPVTSIDGVFHLKDINLSSIAEMQLLAPFGM
GNAKPKVMIKDANIAAMRKIGSEQNHIKVTIENDGSSLDGIGFGLGHLHEHISPYSKLSVIGELSINEWNNIKKPQIFLQ
DMAVNSWQLFDIRSLKRLERLQSVIPEANTTWLFFNEEYISKYKPFAGENMVKITSAEDAEDIKIDGSNVVLADLPPSKE
ILAQVFSGKKPARIYAHFYKEDSDFFSTLPTRDHFKWYFALIAKKGSIDIRRHGNDIAKHKGWSRETVDFMSQVFFELEF
VKINNGVISLNNTSTKRDLADSQTYQLKQAQYTLESDLLYSSFQQLKDWFDEVMQGAVKLEEAKEEKWT

Nucleotide


Download         Length: 2370 bp        

>NTDB_id=616590 LG298_RS20695 WP_250800986.1 4081274..4083643(-) (recJ) [Cytobacillus firmus strain ABH-509]
ATGTTACATTCAAGAACGCGATGGATTGTTCGGCAAACAGAGGAGGATAAAGTACAGCGGCTTTCAAAGGAACTGAATAT
TTCTCCTCTTGCAGCATCGCTTTTAATTAATCGCGGAATGGATACCGTTGATTCAGCACGGTATTTTCTGATGGATAATG
AGCAGGAATTTCATGATCCTTTTTTAATGACAGATATGGCAATTGCTGTTTCCAGAATAAAAGAAGCTATTGAAAAACAG
GAACCTATTTTAATTTTTGGTGATTATGATGCGGATGGTGTGACCAGCACTTCTGTCATGGTGATGGCACTTAAAGATCT
CGGTGCTGCTGTCCAATTCTACATACCGAATCGTTTTACGGAGGGCTATGGGCCAAATATCCCGGCTTTTGAACATGCAG
CCGAAATTGGCATCCGTTTAATTATTACTGTAGATACTGGAATCTCTGCTGTAAATGAAGCAAAAGCTGCAAAAGAGCTT
GGAATGGATTTGATCATTACAGATCACCATGAGCCCGGCCCCGAGCTTCCTGAAGCATTGGCCATTGTGCATCCTAAGCA
TCCGCAGAGCAGATACCCGTTCCGGGAACTTGCCGGTGTTGGGGTAGCATTTAAGCTTGCACATGCGCTTTATGGTGAAA
TGCCTGAACACCTTTTGGAGATAGCTGCGATCGGCACAATTGCTGATCTTGTTTCTTTAACAGGAGAAAACAGACTTATT
GCCAAGCGCGGGATTCAAAAGCTGAAATCAACTAAAAATACAGGTTTGAATGCTTTGTTTAAAGCGGCACGAATCGAGCT
TCCATCCATTAGTGAGGAAACGATCGGCTTTACAATCGGCCCTCGCCTCAATGCAGCAGGAAGGCTTGAAAGCGCGGATC
CTGCTGTGGATCTGCTTTTGACAAATGATCCTTATGAAGCACAAGCCATTGCCGAAGAAATTGAAATGCTGAATAAAGAA
AGGCAATCTATTGTCAATGACATTGCAGCGCAAGCGGTTCATGAGGTTGAAACAAATTTCCCAATCAGTGAGAATTCAGT
TATTGTTGTCGGGAAAGAAGGCTGGAACGCCGGGGTAATCGGAATAGTAGCTTCGAAGCTTGTCGAAAAGTTTTACCGCC
CGGCCATTGTCCTTAGTTATGACAGTGAAAAAGGGCTGGCGAAGGGTTCAGCAAGAAGTATAGAAGGTTTTGATCTGTTC
AAGAATCTTTCTGAATGCCGGGATATCCTGCCTCACTTTGGAGGTCACCCGATGGCAGCGGGTATGACTTTATCCATTGA
TTCGGTTGATGATCTGAGAAGCAGACTGAATACTCTTGCAAATGAACAGCTGAGTGAGGAAGATTTTATTCCTGTAACCT
CTATTGATGGGGTTTTCCATTTAAAAGATATCAATCTTTCTTCCATTGCGGAAATGCAGCTGCTTGCTCCATTCGGGATG
GGAAATGCGAAACCAAAAGTAATGATAAAAGATGCTAATATTGCAGCTATGAGAAAGATCGGATCTGAACAGAACCATAT
TAAAGTCACAATCGAGAATGATGGATCTTCACTTGATGGCATCGGTTTTGGACTTGGGCATTTGCATGAACATATTTCAC
CATACTCCAAGCTGTCCGTTATTGGTGAATTATCCATTAATGAATGGAATAATATTAAAAAACCGCAAATTTTCCTGCAG
GATATGGCAGTCAACTCCTGGCAGCTCTTTGATATCCGGAGTTTAAAACGGCTTGAAAGGCTCCAAAGTGTCATTCCTGA
GGCAAACACAACCTGGTTATTTTTTAATGAGGAATATATTTCTAAATATAAACCATTTGCAGGGGAAAACATGGTGAAAA
TAACATCTGCAGAAGACGCAGAAGACATAAAAATTGATGGCTCAAATGTTGTACTGGCTGATTTGCCGCCTTCAAAGGAA
ATTCTTGCACAGGTTTTCTCCGGAAAAAAACCTGCCAGAATTTATGCTCATTTCTATAAAGAAGACAGTGACTTTTTTAG
CACATTACCAACAAGAGACCATTTTAAATGGTACTTTGCCCTGATTGCTAAAAAAGGTTCAATTGATATCAGGCGTCATG
GGAATGATATAGCGAAGCATAAAGGATGGAGCAGAGAGACAGTAGATTTTATGTCTCAAGTGTTTTTTGAATTGGAATTT
GTTAAAATAAATAATGGTGTCATTTCGTTAAACAATACATCCACTAAACGTGATTTAGCTGATTCACAAACTTATCAGCT
TAAACAGGCACAATACACTCTTGAGAGCGATTTGCTCTATTCATCCTTTCAGCAGCTCAAAGACTGGTTTGATGAAGTTA
TGCAAGGGGCTGTAAAACTTGAGGAGGCAAAGGAAGAAAAATGGACTTAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(314-438)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.284

98.352

0.544