Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LG951_RS12850 Genome accession   NZ_CP085037
Coordinates   2483286..2485610 (-) Length   774 a.a.
NCBI ID   WP_226803773.1    Uniprot ID   -
Organism   Bacillus pumilus strain BIM B-171     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2478286..2490610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG951_RS12835 (LG951_12835) dtd 2479935..2480378 (-) 444 WP_003216527.1 D-aminoacyl-tRNA deacylase -
  LG951_RS12840 (LG951_12840) - 2480397..2482607 (-) 2211 WP_106029087.1 RelA/SpoT family protein -
  LG951_RS12845 (LG951_12845) - 2482750..2483262 (-) 513 WP_003216770.1 adenine phosphoribosyltransferase -
  LG951_RS12850 (LG951_12850) recJ 2483286..2485610 (-) 2325 WP_226803773.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LG951_RS12855 (LG951_12855) - 2485692..2486045 (-) 354 WP_226803774.1 lipopolysaccharide assembly LapA domain-containing protein -
  LG951_RS12860 (LG951_12860) - 2486148..2486636 (-) 489 WP_106035305.1 cation:proton antiporter regulatory subunit -
  LG951_RS12865 (LG951_12865) secDF 2486815..2489043 (-) 2229 WP_003216337.1 protein translocase subunit SecDF -
  LG951_RS12870 (LG951_12870) - 2489085..2489381 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87417.05 Da        Isoelectric Point: 4.9246

>NTDB_id=614829 LG951_RS12850 WP_226803773.1 2483286..2485610(-) (recJ) [Bacillus pumilus strain BIM B-171]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKELSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPTQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEARMLSPFGMQN
PKPFIMIEDVRLEEIRTIGANQNHIKMSLKDEDKLLDCVGFHQGQLKEEVVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKTISTLDPEKYVVVCFDEETKQDVNIPIHHVHAENVSTFDVEGKYVVFADVPADEHLLRQLLE
NQKPARVYTVFQRKEDHFMSSFPSRDQFKWFYGFLLKRGSFPLKEQGMELAKHRGWTKDTMIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSIMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=614829 LG951_RS12850 WP_226803773.1 2483286..2485610(-) (recJ) [Bacillus pumilus strain BIM B-171]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTTGAAGAAGTAGAAGAAGCAAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAGGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGTT
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTCCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACCGTCGATACAGGCATTGCAGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAACTGCCTGAGGCACTTGCGATTGTTCATCCTAAGCA
GCCAGGCTGTGAGTATCCCTTTAAAGAACTTGCCGGTGTAGGCGTAGCATTTAAAGTGGCGCATGCACTTCTTGGAAAGC
TGCCAACTCAGCTGTTAGATCTTGCGGCAATTGGGACAATAGCGGATCTTGTGCCGCTTCATGATGAGAATAGATGGCTC
GCTAAAAAAGGGCTTATGCAGCTTAGGCAGTCGAATAGACCGGGGCTGAAGGCGTTATTGAAAGAAGCAGGTGCTACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGGCGAATTGAACAGGCCGATC
CAGCTGTTCACCTTCTCATGACAGAGGATAAGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAACTGAATAAAGAA
CGTCAAAAAATTGTGAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGACAGGTGATGACCAGTCTGCAATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCGAGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAGTGCCGCGACATTCTGCCGCATTTTGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGGGCGGATGATGT
TCCTTTACTAAGGGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAAGAAGATTTTATTCCGATTCAGCGGGTTG
ATGCTGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGCGAGGATGCTGTCACCATTTGGCATGCAGAAT
CCAAAGCCGTTCATCATGATTGAAGATGTGAGACTTGAAGAAATCCGAACAATTGGAGCAAATCAGAATCATATTAAAAT
GTCTCTGAAAGATGAAGATAAGCTGCTTGACTGTGTCGGTTTTCATCAAGGTCAATTAAAAGAAGAAGTTGTCACAGGTA
GCCGCATTTCCGTCATTGGTGAAATGTCCATCAATGAATGGAATAACCGGAAGAAACCACAACTCATGCTAAAAGATGCC
CGAGTAGATGAATGGCAGCTGTTTGATTTGCGCGGCAAAAAGGATTGGGAAAAGACCATCTCCACGCTTGATCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGAAACAAAACAGGATGTCAATATCCCGATTCATCATGTCCATGCAGAAAATGTTT
CAACATTCGATGTAGAAGGCAAGTATGTTGTCTTTGCAGATGTTCCTGCAGATGAACACTTGCTAAGGCAGCTGTTAGAA
AACCAAAAGCCAGCTAGAGTTTATACGGTTTTCCAAAGAAAAGAAGATCATTTCATGTCCTCTTTTCCTAGCCGCGATCA
ATTCAAATGGTTTTATGGATTCTTATTAAAACGAGGAAGTTTCCCTCTCAAGGAGCAAGGAATGGAGCTTGCGAAACATA
GAGGCTGGACAAAAGATACAATGATTTTTATGACAAAGGTGTTTTTTGAACTTGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACAGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATAATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(309-437)

DHH family (DHH)

(83-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.181

100

0.674