Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   NLS69_RS24695 Genome accession   NZ_CP100653
Coordinates   5271212..5271637 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain F13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5266212..5276637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLS69_RS24680 (NLS69_24680) pilX 5266782..5267369 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  NLS69_RS24685 (NLS69_24685) pilY1 5267381..5270866 (+) 3486 WP_034000905.1 type 4a pilus biogenesis protein PilY1 -
  NLS69_RS24690 (NLS69_24690) pilY2 5270868..5271215 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  NLS69_RS24695 (NLS69_24695) comF 5271212..5271637 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  NLS69_RS24700 (NLS69_24700) ispH 5271684..5272628 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  NLS69_RS24705 (NLS69_24705) fkpB 5272714..5273154 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  NLS69_RS24710 (NLS69_24710) lspA 5273147..5273656 (-) 510 WP_003102615.1 signal peptidase II -
  NLS69_RS24715 (NLS69_24715) ileS 5273649..5276480 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=605980 NLS69_RS24695 WP_003094721.1 5271212..5271637(+) (comF) [Pseudomonas aeruginosa strain F13]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=605980 NLS69_RS24695 WP_003094721.1 5271212..5271637(+) (comF) [Pseudomonas aeruginosa strain F13]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGTC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383