Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K8P00_RS00750 Genome accession   NZ_CP082861
Coordinates   149347..150489 (-) Length   380 a.a.
NCBI ID   WP_223417333.1    Uniprot ID   -
Organism   Eikenella corrodens strain FDAARGOS_1474     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 144347..155489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8P00_RS00730 (K8P00_00730) ndk 144711..145136 (-) 426 WP_003823678.1 nucleoside-diphosphate kinase -
  K8P00_RS00735 (K8P00_00735) - 145344..146156 (-) 813 WP_003823676.1 sulfite exporter TauE/SafE family protein -
  K8P00_RS00740 (K8P00_00740) pssA 146368..147156 (-) 789 WP_003823673.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  K8P00_RS00745 (K8P00_00745) lpdA 147399..149201 (+) 1803 WP_003823670.1 dihydrolipoyl dehydrogenase -
  K8P00_RS00750 (K8P00_00750) pilU 149347..150489 (-) 1143 WP_223417333.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8P00_RS00755 (K8P00_00755) - 150615..151058 (-) 444 WP_003823668.1 universal stress protein -
  K8P00_RS00760 (K8P00_00760) mtgA 151319..152089 (-) 771 WP_003823666.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  K8P00_RS00765 (K8P00_00765) - 152272..153216 (-) 945 WP_003823664.1 LysR substrate-binding domain-containing protein -
  K8P00_RS00770 (K8P00_00770) - 153660..154352 (+) 693 WP_003823661.1 OmpA family protein -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42053.22 Da        Isoelectric Point: 6.2860

>NTDB_id=603192 K8P00_RS00750 WP_223417333.1 149347..150489(-) (pilU) [Eikenella corrodens strain FDAARGOS_1474]
MKKFIHGLLNHLVQSKGSDLFITTGFPPAMKLDGKLTPITDKPLTADHTALIARALMDDKQAEEFDNTKECNFAISLAGV
SRFRINAMVQRGAAALVCRVITSQIPKFDNMNLPPILKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDYRNENSHGHIITI
EDPIEFVHPHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMATLHANNSNQALD
RIINFFPEERRTQLLNDLSLNLKGFISQRLVPKPDGKGRVAAVEVLLNSPLIAELILNGDIHGVKEIMARSRDLGMQTFD
QSLFDLYEAGHIAYEDALKNADSVNDLRLNIQLNSKRGRNNEKSGIDSLAIVGMDEADEA

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=603192 K8P00_RS00750 WP_223417333.1 149347..150489(-) (pilU) [Eikenella corrodens strain FDAARGOS_1474]
ATGAAAAAATTCATCCATGGCCTGCTCAACCACCTGGTGCAGAGCAAAGGCTCCGATCTGTTCATCACCACCGGCTTCCC
CCCGGCCATGAAGCTCGACGGCAAGCTCACCCCGATTACCGACAAACCGCTCACCGCCGACCACACCGCCCTCATCGCCC
GCGCCCTGATGGACGACAAACAGGCCGAAGAATTCGATAACACCAAAGAATGCAACTTCGCCATCAGCCTGGCCGGCGTG
TCCCGCTTCCGCATCAACGCCATGGTTCAGCGCGGTGCCGCCGCCCTCGTGTGCCGTGTGATCACCAGCCAGATTCCCAA
GTTCGACAACATGAACCTGCCGCCGATTTTGAAACAAGTGGTGATGGAAAAACGCGGCCTCGTGATTTTCGTGGGCGGTA
CCGGCTCCGGCAAATCCACTTCCCTGGCTGCCATGATCGACTACCGTAACGAAAACAGCCACGGCCACATCATCACCATC
GAAGACCCGATCGAATTCGTGCACCCGCACAAAAACTGCATCATCACCCAGCGCGAAGTGGGCGTGGACACCGAAAACTG
GTTTGCCGCCCTCAAAAACACCCTGCGCCAAGCCCCCGACGTGATCCTCATCGGCGAAATCCGCGACCGCGAAACCATGG
ACTACGCCCTGGCCTTCGCCGAAACCGGCCACCTCTGCATGGCCACCCTACACGCCAACAACTCCAACCAAGCGCTCGAC
CGCATCATCAACTTCTTCCCCGAAGAGCGCCGTACCCAGCTGTTGAATGACCTCTCGCTCAACCTCAAAGGCTTCATTTC
CCAGCGCCTCGTGCCCAAGCCCGACGGCAAAGGCCGCGTGGCCGCCGTGGAAGTGCTGCTCAACTCCCCGCTGATTGCCG
AGCTGATTTTGAATGGCGACATCCACGGCGTGAAAGAAATCATGGCCCGCTCCCGCGACTTAGGCATGCAAACCTTCGAC
CAATCCCTGTTCGACCTCTACGAAGCCGGCCACATTGCCTACGAAGACGCCCTGAAAAACGCCGACTCGGTGAATGATTT
GCGCCTCAACATCCAGCTCAACAGCAAACGCGGCCGCAACAACGAAAAATCCGGCATCGACAGCCTAGCCATCGTCGGCA
TGGATGAAGCTGACGAAGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.318

91.842

0.563
  pilU Acinetobacter baylyi ADP1

59.143

92.105

0.545
  pilU Vibrio cholerae strain A1552

55.307

94.211

0.521
  pilT Legionella pneumophila strain Lp02

42.229

89.737

0.379
  pilT Legionella pneumophila strain ERS1305867

42.229

89.737

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.771

87.368

0.374
  pilT Pseudomonas aeruginosa PAK

40.653

88.684

0.361
  pilT Acinetobacter baylyi ADP1

40.413

89.211

0.361