Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   NHZ69_RS23380 Genome accession   NZ_CP099797
Coordinates   5045982..5046407 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PAO1-N     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5040982..5051407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHZ69_RS23365 (NHZ69_23365) pilX 5041552..5042139 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  NHZ69_RS23370 (NHZ69_23370) pilY1 5042151..5045636 (+) 3486 WP_003115287.1 type 4a pilus biogenesis protein PilY1 -
  NHZ69_RS23375 (NHZ69_23375) pilY2 5045638..5045985 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  NHZ69_RS23380 (NHZ69_23380) comF 5045982..5046407 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  NHZ69_RS23385 (NHZ69_23385) ispH 5046454..5047398 (-) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  NHZ69_RS23390 (NHZ69_23390) fkpB 5047484..5047924 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  NHZ69_RS23395 (NHZ69_23395) lspA 5047917..5048426 (-) 510 WP_003112823.1 signal peptidase II -
  NHZ69_RS23400 (NHZ69_23400) ileS 5048419..5051250 (-) 2832 WP_003112822.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=602873 NHZ69_RS23380 WP_003094721.1 5045982..5046407(+) (comF) [Pseudomonas aeruginosa strain PAO1-N]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=602873 NHZ69_RS23380 WP_003094721.1 5045982..5046407(+) (comF) [Pseudomonas aeruginosa strain PAO1-N]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383