Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   K7B13_RS05195 Genome accession   NZ_CP082278
Coordinates   1046197..1049697 (+) Length   1166 a.a.
NCBI ID   WP_115997927.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain 35M     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1041197..1054697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7B13_RS05170 (K7B13_05170) - 1041362..1042519 (-) 1158 WP_212100860.1 AbrB family transcriptional regulator -
  K7B13_RS05175 (K7B13_05175) - 1042685..1043881 (-) 1197 WP_212100863.1 MFS transporter -
  K7B13_RS05180 (K7B13_05180) - 1043897..1044517 (-) 621 WP_115997925.1 TetR/AcrR family transcriptional regulator -
  K7B13_RS05185 (K7B13_05185) - 1044744..1045271 (-) 528 WP_088462317.1 ferritin-like domain-containing protein -
  K7B13_RS05190 (K7B13_05190) - 1045342..1046055 (-) 714 WP_115997926.1 DUF421 domain-containing protein -
  K7B13_RS05195 (K7B13_05195) addB 1046197..1049697 (+) 3501 WP_115997927.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  K7B13_RS05200 (K7B13_05200) addA 1049684..1053388 (+) 3705 WP_212101650.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  K7B13_RS05205 (K7B13_05205) sbcD 1053455..1054627 (+) 1173 WP_065981123.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133691.29 Da        Isoelectric Point: 5.2756

>NTDB_id=600842 K7B13_RS05195 WP_115997927.1 1046197..1049697(+) (addB) [Bacillus amyloliquefaciens strain 35M]
MGVEFLVGRSGSGKTRLIIDSIQEELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADIIKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQHVPELACIESQYDVRPAAVYTGGQEAFTVIQAQNR
RAELEGIAREIQSLVRDRGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREW
MAEPLIRLQNRLKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQQAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEM
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHERAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYDAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMELDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=600842 K7B13_RS05195 WP_115997927.1 1046197..1049697(+) (addB) [Bacillus amyloliquefaciens strain 35M]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGAAGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGCTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCACACGGGG
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAACTGATTGAAGAACATAAGCACGATTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGATTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GCATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATACCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCATTATTTGCATTCAGAGGATTATTT
GACACTGCTCGCTCAGCAGATTCCTTTGGCGGATATTATAAAAGGCGCCCGTGTTTATGTCGACGGCTTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTCGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCTCTGACGGCGGACAGCCCT
TCGGCGGAACAGGCTCCGGATGAGCTCGACCTGTTCAGAATGACCGGCAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAGTTGAATGCCGATATTTCCTGCAAGGAATTGCATGGAACGAAGCGGCATCAGCATGTGCCGGAGCTTGCCTGTATTG
AATCGCAGTACGATGTGCGTCCCGCGGCTGTGTATACTGGCGGACAGGAAGCTTTTACCGTCATACAGGCGCAGAACAGA
AGAGCTGAACTCGAGGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACCGAGGATACCGGTACAGAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGTTGAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATAGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGCTCAAGCTTGGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCCCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTTGA
TCAGCTTGAAAACTACTGTATCGCCTACGGAATCAAAGGTGAGCGCTGGACGAACGGGGAGCGGTTTGTATACAGGCGGT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAATGAGACGAGAGAGTGG
ATGGCCGAGCCGCTCATCCGGCTTCAAAACCGGCTGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAAGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGATCGGAAAAGACAGCAGGCCGAAGAGGCCGGGAACATGATTGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAAGAGTTTGCGGGAATGATGGGTGAAGATGAAATG
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCGCTTCATTTTTCTTTAATTCCTCCTGCGCTTGACCA
AGTATTTGTAGGGAATATGGATCTGTCCAGAATGTACGGCACCTCCTGCACGTTTGTTATCGGGGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCGGTCGGAGTGGAGCTTTCT
TCCGCTGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GACATATCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATTGTTGTAAACAGGCTCGGCGAGCTGTTTCCCG
ATCATCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAACAGCTTCAGTATCTCGTCAATAAACAG
GTGGCGCAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTGTGGTGGAGCGCATA
CAACGTGCTGATGAAAGAGCCTGATCACGAGCGTGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGAAATGAGGCAAAGC
GTCTTGAACGGCCTGTTTCGCGACAGCTTTACGGCGAGCACATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCTCATTTTGCATCTCACGGACTGCAATTAAAAGAGCGGCAGTTTTTTAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTTTCCTATGATGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGGCACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTCTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGATCGGCCTTGAACTCGGTTTTGGCGGAAGCGGACCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGCTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGACAAGGGGCTTGATCTCGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACATATCTTGA
CTTATCAATTACACACTCAGCGGATTGGCTCGGCATGAAAGCGACACCGGCAGGCGTTTTGTATTTCCATATTCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGAACTTGATGAAATCGAGCAGGAAATCTTTAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGCCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATTATCAATGCCGGTTT
GAAAAAGGACGGTTCTTTACGGTCCGATTCAGCGGCGGTCAGCGAACGGGATTTTCATCTCTTAACAGACCATGTGAGGC
GGACATTTGAGCAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAATCGGTGTGCCAGTTTGATGAGTCGCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACTATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(307-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.19

100

0.832