Detailed information NTG0600571

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: K4A81_RS13910
• Genome accession: NZ_CP082262
• Coordinates: 2801610..2803988 (-)
• Length: 792 a.a.
• NCBI ID: WP_014418522.1
• Uniprot ID: I2C8C6
• Organism: Bacillus velezensis strain BIM B-454D
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2796610..2808988

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K4A81_RS13875 (K4A81_13875)	-	2797682..2798410 (+)	729	WP_007408209.1	amino acid ABC transporter ATP-binding protein	-
K4A81_RS13880 (K4A81_13880)	-	2798431..2799258 (+)	828	WP_021494374.1	transporter substrate-binding domain-containing protein	-
K4A81_RS13885 (K4A81_13885)	-	2799315..2799965 (+)	651	WP_100261915.1	amino acid ABC transporter permease	-
K4A81_RS13890 (K4A81_13890)	-	2799984..2800640 (+)	657	WP_100261916.1	amino acid ABC transporter permease	-
K4A81_RS13895 (K4A81_13895)	-	2800676..2800807 (-)	132	WP_003152741.1	YrzQ family protein	-
K4A81_RS13900 (K4A81_13900)	-	2800829..2801020 (-)	192	WP_003152738.1	hypothetical protein	-
K4A81_RS13905 (K4A81_13905)	-	2801033..2801491 (-)	459	WP_021494376.1	PRC-barrel domain-containing protein	-
K4A81_RS13910 (K4A81_13910)	recD2	2801610..2803988 (-)	2379	WP_014418522.1	ATP-dependent RecD-like DNA helicase	Machinery gene
K4A81_RS13915 (K4A81_13915)	-	2804007..2804627 (-)	621	WP_088461189.1	lipopolysaccharide assembly protein LapB	-
K4A81_RS13920 (K4A81_13920)	mnmA	2804715..2805830 (-)	1116	WP_012118077.1	tRNA 2-thiouridine(34) synthase MnmA	-
K4A81_RS13925 (K4A81_13925)	-	2805867..2807006 (-)	1140	WP_007612815.1	cysteine desulfurase family protein	-
K4A81_RS13930 (K4A81_13930)	cymR	2807025..2807441 (-)	417	WP_003152730.1	cysteine metabolism transcriptional regulator CymR	-
K4A81_RS13935 (K4A81_13935)	-	2807636..2808904 (+)	1269	WP_017418226.1	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88412.10 Da
• Isoelectric Point: 4.9490

>NTDB_id=600571 K4A81_RS13910 WP_014418522.1 2801610..2803988(-) (recD2) [Bacillus velezensis strain BIM B-454D]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILTDASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDETFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQMKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=600571 K4A81_RS13910 WP_014418522.1 2801610..2803988(-) (recD2) [Bacillus velezensis strain BIM B-454D]
GTGGACCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCATAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAAGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTACGGAAAAACCGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGGATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTCGGAGACGGCGCGCTCAATAAAATTCTGACGGATGCGTCGGTGCTTT
ACGATGTGCCGAAAATGTCGAAAAAGAAAGCGGATAAGCTTGCCGGAGAGCTGCAGCGGCATCAGGGGCTTGAGCAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCAATGAAAATATATCAGGCCTACGAAACGGAAACCCTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGTTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAA
GGCCATACGTACATAGAGACAAAGCAGCTGATTATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
CACGGAAATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATAGAAGGAGAGCGCTGCTATT
TTCCGTCCTTGTTTTACGCCGAACAAAATGTCGCAAAACGCGTGCTCGACATTGCGGCTCAAACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTTGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTACTGACCGGAGGACCCGGGACGGGGAAAACGACCGTTATTAAAG
GAATCGTAGAATTATACAGTGAACTGCACGGAGTATCGCTTGACCCCGGTGATTATAAAAAAGACGAGACGTTCCCGGTG
CTGCTTGCCGCACCGACGGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGGCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTACAATGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTCGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGACCTTTTGGCTTCTGAGGTGCTGCCGACCGTGCGTCT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCGCATGATATGAAAAAAGGACTGCTTCCAAAGG
GGCTGACCGCGCCGACCAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATGAAAGAAGTCGTGGATCAGGTCGTC
TCGAACGCGTTAAAAAAGGGGTATACCTCCAGGGATATTCAAGTGCTCGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAAGACATCATGAATCCGCCGAAAGAAAAAAGGCGGGAGCTGAAATTCGGTGATGTCGTCT
ACAGGACGGGCGATAAAATATTACAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAACGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGAAGCGAATTCCCGATTGTCG
TCCTGCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATAACAAGAGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACCGTCAGACAGACGTCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATATG
ATGCGAACATCGGAATGGAAGGCATCACGCCGTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

• AAA domain (AAA_30) (341-548)

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

3D structure

• AlphaFold DB: I2C8C6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	88.622	99.874	0.885