Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBY18_RS01525 Genome accession   NZ_CP098206
Coordinates   327539..329035 (+) Length   498 a.a.
NCBI ID   WP_021524783.1    Uniprot ID   -
Organism   Escherichia coli strain Z0117EC0054     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 322539..334035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY18_RS01495 (NBY18_01495) tusA 322619..322864 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  NBY18_RS01500 (NBY18_01500) zntA 323133..325331 (-) 2199 WP_000106588.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NBY18_RS01505 (NBY18_01505) yhhN 325405..326031 (-) 627 WP_000964729.1 lysoplasmalogenase -
  NBY18_RS01510 (NBY18_01510) yhhM 326172..326531 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  NBY18_RS01515 (NBY18_01515) yhhL 326534..326803 (-) 270 WP_001295207.1 DUF1145 family protein -
  NBY18_RS01520 (NBY18_01520) rsmD 326793..327389 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NBY18_RS01525 (NBY18_01525) pilA 327539..329035 (+) 1497 WP_021524783.1 signal recognition particle-docking protein FtsY Machinery gene
  NBY18_RS01530 (NBY18_01530) ftsE 329038..329706 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  NBY18_RS01535 (NBY18_01535) ftsX 329699..330757 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NBY18_RS01540 (NBY18_01540) rpoH 331002..331856 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  NBY18_RS01545 (NBY18_01545) dapA 331960..332844 (-) 885 WP_000661262.1 4-hydroxy-tetrahydrodipicolinate synthase -
  NBY18_RS01550 (NBY18_01550) - 332844..333764 (-) 921 WP_001332164.1 phosphoglycerate dehydrogenase -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54555.35 Da        Isoelectric Point: 4.1948

>NTDB_id=597972 NBY18_RS01525 WP_021524783.1 327539..329035(+) (pilA) [Escherichia coli strain Z0117EC0054]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAGTFAANVVEVTEQVAE
SEKAQPEAEVVAQPESVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNGVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=597972 NBY18_RS01525 WP_021524783.1 327539..329035(+) (pilA) [Escherichia coli strain Z0117EC0054]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGGAACTTTTGCTGCCAACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAATCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
TGGGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

44.667

90.361

0.404