Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   M8341_RS30050 Genome accession   NZ_CP097709
Coordinates   6401765..6402361 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain PA-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6396765..6407361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8341_RS30030 (M8341_30025) aroK 6397896..6398414 (-) 519 WP_003095833.1 shikimate kinase AroK -
  M8341_RS30035 (M8341_30030) pilQ 6398426..6400570 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  M8341_RS30040 (M8341_30035) comL/pilP 6400624..6401148 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  M8341_RS30045 (M8341_30040) pilO 6401145..6401768 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  M8341_RS30050 (M8341_30045) pilN 6401765..6402361 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  M8341_RS30055 (M8341_30050) pilM 6402361..6403425 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  M8341_RS30060 (M8341_30055) - 6403610..6406078 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=595850 M8341_RS30050 WP_003095839.1 6401765..6402361(-) (pilN) [Pseudomonas aeruginosa strain PA-1]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=595850 M8341_RS30050 WP_003095839.1 6401765..6402361(-) (pilN) [Pseudomonas aeruginosa strain PA-1]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGTCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATTGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369