Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   M8341_RS27445 Genome accession   NZ_CP097709
Coordinates   5823292..5823717 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PA-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5818292..5828717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8341_RS27430 (M8341_27425) pilX 5818856..5819443 (+) 588 WP_034037187.1 type 4a pilus minor pilin PilX -
  M8341_RS27435 (M8341_27430) pilY1 5819455..5822946 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  M8341_RS27440 (M8341_27435) pilY2 5822948..5823295 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  M8341_RS27445 (M8341_27440) comF 5823292..5823717 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  M8341_RS27450 (M8341_27445) ispH 5823764..5824708 (-) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  M8341_RS27455 (M8341_27450) fkpB 5824794..5825234 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  M8341_RS27460 (M8341_27455) lspA 5825227..5825736 (-) 510 WP_003102615.1 signal peptidase II -
  M8341_RS27465 (M8341_27460) ileS 5825729..5828560 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=595842 M8341_RS27445 WP_003094721.1 5823292..5823717(+) (comF) [Pseudomonas aeruginosa strain PA-1]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=595842 M8341_RS27445 WP_003094721.1 5823292..5823717(+) (comF) [Pseudomonas aeruginosa strain PA-1]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383