Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   M8Z32_RS26685 Genome accession   NZ_CP097555
Coordinates   5755204..5755800 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain B1.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5750204..5760800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8Z32_RS26665 (M8Z32_26635) aroK 5751335..5751853 (-) 519 WP_034076693.1 shikimate kinase AroK -
  M8Z32_RS26670 (M8Z32_26640) pilQ 5751865..5754009 (-) 2145 WP_012614629.1 type 4a pilus secretin PilQ Machinery gene
  M8Z32_RS26675 (M8Z32_26645) comL/pilP 5754063..5754587 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  M8Z32_RS26680 (M8Z32_26650) pilO 5754584..5755207 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  M8Z32_RS26685 (M8Z32_26655) pilN 5755204..5755800 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  M8Z32_RS26690 (M8Z32_26660) pilM 5755800..5756864 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  M8Z32_RS26695 (M8Z32_26665) - 5757049..5759520 (+) 2472 WP_043089239.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=595156 M8Z32_RS26685 WP_003095839.1 5755204..5755800(-) (pilN) [Pseudomonas aeruginosa strain B1.2]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=595156 M8Z32_RS26685 WP_003095839.1 5755204..5755800(-) (pilN) [Pseudomonas aeruginosa strain B1.2]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGATTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369