Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   M8Z32_RS24040 Genome accession   NZ_CP097555
Coordinates   5169847..5170272 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain B1.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5164847..5175272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8Z32_RS24025 (M8Z32_24000) pilX 5165411..5165998 (+) 588 WP_015648054.1 type 4a pilus minor pilin PilX -
  M8Z32_RS24030 (M8Z32_24005) pilY1 5166010..5169501 (+) 3492 WP_010793785.1 type 4a pilus biogenesis protein PilY1 -
  M8Z32_RS24035 (M8Z32_24010) pilY2 5169503..5169850 (+) 348 WP_021264112.1 type 4a fimbrial biogenesis protein PilY2 -
  M8Z32_RS24040 (M8Z32_24015) comF 5169847..5170272 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  M8Z32_RS24045 (M8Z32_24020) ispH 5170319..5171263 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  M8Z32_RS24050 (M8Z32_24025) fkpB 5171349..5171789 (-) 441 WP_043089468.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  M8Z32_RS24055 (M8Z32_24030) lspA 5171782..5172291 (-) 510 WP_003094728.1 signal peptidase II -
  M8Z32_RS24060 (M8Z32_24035) ileS 5172284..5175115 (-) 2832 WP_031689113.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=595148 M8Z32_RS24040 WP_003094721.1 5169847..5170272(+) (comF) [Pseudomonas aeruginosa strain B1.2]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=595148 M8Z32_RS24040 WP_003094721.1 5169847..5170272(+) (comF) [Pseudomonas aeruginosa strain B1.2]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383