Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   M2I99_RS27510 Genome accession   NZ_CP096960
Coordinates   5814257..5814682 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain NY11254     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5809257..5819682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2I99_RS27495 (M2I99_27430) pilX 5809821..5810408 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  M2I99_RS27500 (M2I99_27435) pilY1 5810420..5813911 (+) 3492 WP_003094704.1 type 4a pilus biogenesis protein PilY1 -
  M2I99_RS27505 (M2I99_27440) pilY2 5813913..5814260 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  M2I99_RS27510 (M2I99_27445) comF 5814257..5814682 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  M2I99_RS27515 (M2I99_27450) ispH 5814729..5815673 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  M2I99_RS27520 (M2I99_27455) fkpB 5815759..5816199 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  M2I99_RS27525 (M2I99_27460) lspA 5816192..5816701 (-) 510 WP_003094728.1 signal peptidase II -
  M2I99_RS27530 (M2I99_27465) ileS 5816694..5819525 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=592934 M2I99_RS27510 WP_003094721.1 5814257..5814682(+) (comF) [Pseudomonas aeruginosa strain NY11254]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=592934 M2I99_RS27510 WP_003094721.1 5814257..5814682(+) (comF) [Pseudomonas aeruginosa strain NY11254]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383