Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   M2I92_RS26535 Genome accession   NZ_CP096945
Coordinates   5521881..5522306 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain NY5525     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5516881..5527306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2I92_RS26520 (M2I92_26440) pilX 5517451..5518038 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  M2I92_RS26525 (M2I92_26445) pilY1 5518050..5521535 (+) 3486 WP_034040518.1 type 4a pilus biogenesis protein PilY1 -
  M2I92_RS26530 (M2I92_26450) pilY2 5521537..5521884 (+) 348 WP_003161774.1 type 4a fimbrial biogenesis protein PilY2 -
  M2I92_RS26535 (M2I92_26455) comF 5521881..5522306 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  M2I92_RS26540 (M2I92_26460) ispH 5522353..5523297 (-) 945 WP_034040515.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  M2I92_RS26545 (M2I92_26465) fkpB 5523383..5523823 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  M2I92_RS26550 (M2I92_26470) lspA 5523816..5524325 (-) 510 WP_003102615.1 signal peptidase II -
  M2I92_RS26555 (M2I92_26475) ileS 5524318..5527149 (-) 2832 WP_023103515.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=592323 M2I92_RS26535 WP_003094721.1 5521881..5522306(+) (comF) [Pseudomonas aeruginosa strain NY5525]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=592323 M2I92_RS26535 WP_003094721.1 5521881..5522306(+) (comF) [Pseudomonas aeruginosa strain NY5525]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383