Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   M1Q10_RS00745 Genome accession   NZ_CP096813
Coordinates   156332..156757 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 8D     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 151332..161757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1Q10_RS00730 (M1Q10_00730) pilX 151896..152483 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  M1Q10_RS00735 (M1Q10_00735) pilY1 152495..155986 (+) 3492 WP_010793785.1 type 4a pilus biogenesis protein PilY1 -
  M1Q10_RS00740 (M1Q10_00740) pilY2 155988..156335 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  M1Q10_RS00745 (M1Q10_00745) comF 156332..156757 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  M1Q10_RS00750 (M1Q10_00750) ispH 156804..157748 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  M1Q10_RS00755 (M1Q10_00755) fkpB 157834..158274 (-) 441 WP_003161775.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  M1Q10_RS00760 (M1Q10_00760) lspA 158267..158776 (-) 510 WP_003102615.1 signal peptidase II -
  M1Q10_RS00765 (M1Q10_00765) ileS 158769..161600 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=590580 M1Q10_RS00745 WP_003094721.1 156332..156757(+) (comF) [Pseudomonas aeruginosa strain 8D]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=590580 M1Q10_RS00745 WP_003094721.1 156332..156757(+) (comF) [Pseudomonas aeruginosa strain 8D]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383