Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   EGT72_RS14195 Genome accession   NZ_CP079792
Coordinates   2853815..2854258 (-) Length   147 a.a.
NCBI ID   WP_171428529.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ_082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2848815..2859258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT72_RS14160 (EGT72_014160) tnpB 2849095..2849406 (+) 312 WP_267461040.1 IS66 family insertion sequence element accessory protein TnpB -
  EGT72_RS14165 (EGT72_014165) - 2849481..2851085 (+) 1605 WP_201701530.1 IS66 family transposase -
  EGT72_RS14170 (EGT72_014170) - 2851117..2851491 (+) 375 Protein_2752 lipase -
  EGT72_RS14175 (EGT72_014175) rplS 2851551..2851925 (-) 375 WP_004691454.1 50S ribosomal protein L19 -
  EGT72_RS14180 (EGT72_014180) trmD 2852066..2852806 (-) 741 WP_125280918.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  EGT72_RS14185 (EGT72_014185) rimM 2852834..2853382 (-) 549 WP_004978035.1 ribosome maturation factor RimM -
  EGT72_RS14190 (EGT72_014190) rpsP 2853408..2853665 (-) 258 WP_004718284.1 30S ribosomal protein S16 -
  EGT72_RS14195 (EGT72_014195) comF 2853815..2854258 (-) 444 WP_171428529.1 type IV pilin protein Machinery gene
  EGT72_RS14200 (EGT72_014200) - 2854252..2854767 (-) 516 WP_125280916.1 type IV pilin protein -
  EGT72_RS14205 (EGT72_014205) - 2854777..2858949 (-) 4173 WP_125280915.1 PilC/PilY family type IV pilus protein -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 16163.29 Da        Isoelectric Point: 8.4362

>NTDB_id=589530 EGT72_RS14195 WP_171428529.1 2853815..2854258(-) (comF) [Acinetobacter johnsonii strain AJ_082]
MVMSRNLGFTLIELMIVVAIIAILAAIAYPSYTSYRVKTNRVDVQAQMMNIAQRLQSYKSVNHSYTGATLAGLGISATYP
NSGEYTLNLAIDADNQGYLLKAKPNSSTVQKNNGEICLNQDGFKFWQKGSDCDAGNLSNQSSWDTRH

Nucleotide


Download         Length: 444 bp        

>NTDB_id=589530 EGT72_RS14195 WP_171428529.1 2853815..2854258(-) (comF) [Acinetobacter johnsonii strain AJ_082]
ATGGTAATGAGCAGAAATTTAGGCTTTACGCTCATTGAGTTGATGATAGTGGTGGCGATTATTGCAATTCTAGCCGCTAT
AGCTTATCCCTCTTATACATCTTATAGAGTAAAAACAAATCGTGTGGATGTTCAAGCTCAAATGATGAATATTGCCCAGC
GTTTACAGAGCTATAAATCTGTAAATCATAGTTATACTGGAGCAACTTTAGCAGGTCTTGGTATTTCAGCTACATATCCA
AATAGTGGTGAATATACTTTAAATTTAGCGATTGATGCGGATAATCAAGGGTATTTATTAAAAGCAAAGCCAAATAGTTC
GACAGTTCAAAAAAACAATGGTGAGATTTGTTTAAACCAAGACGGATTTAAGTTTTGGCAAAAGGGCAGTGATTGTGATG
CTGGAAACCTAAGCAACCAATCTTCTTGGGACACTAGACATTAG

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(30-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

63.121

95.918

0.605
  pilE Acinetobacter baumannii D1279779

51.079

94.558

0.483