Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   MW396_RS25190 Genome accession   NZ_CP095538
Coordinates   5171950..5172714 (-) Length   254 a.a.
NCBI ID   WP_001136250.1    Uniprot ID   -
Organism   Escherichia coli strain GN04592     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 5166950..5177714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW396_RS25160 (MW396_25155) - 5167585..5168943 (-) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -
  MW396_RS25165 (MW396_25160) - 5169020..5169301 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  MW396_RS25170 (MW396_25165) - 5169298..5169771 (-) 474 WP_001377099.1 PTS sugar transporter subunit IIA -
  MW396_RS25175 (MW396_25170) - 5169796..5170542 (-) 747 WP_001322918.1 GntR family transcriptional regulator -
  MW396_RS25180 (MW396_25175) nikR 5170741..5171142 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  MW396_RS25185 (MW396_25180) nikE 5171147..5171953 (-) 807 WP_000173693.1 nickel import ATP-binding protein NikE -
  MW396_RS25190 (MW396_25185) amiE 5171950..5172714 (-) 765 WP_001136250.1 nickel import ATP-binding protein NikD Regulator
  MW396_RS25195 (MW396_25190) nikC 5172714..5173547 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  MW396_RS25200 (MW396_25195) nikB 5173544..5174488 (-) 945 WP_000947088.1 nickel ABC transporter permease subunit NikB -
  MW396_RS25205 (MW396_25200) nikA 5174488..5176062 (-) 1575 WP_000493127.1 nickel ABC transporter substrate-binding protein -
  MW396_RS25210 (MW396_25205) acpT 5176173..5176760 (-) 588 WP_000285765.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=583966 MW396_RS25190 WP_001136250.1 5171950..5172714(-) (amiE) [Escherichia coli strain GN04592]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGSSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAALEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=583966 MW396_RS25190 WP_001136250.1 5171950..5172714(-) (amiE) [Escherichia coli strain GN04592]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAACCGCTGGTGCACGGCGTATCGTTAACTCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCAGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCCGCACTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTTCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCTTTAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398