Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   MW400_RS23650 Genome accession   NZ_CP095533
Coordinates   5008766..5009530 (+) Length   254 a.a.
NCBI ID   WP_001136218.1    Uniprot ID   -
Organism   Escherichia coli strain GN02461     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 5003766..5014530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW400_RS23630 (MW400_23620) acpT 5004720..5005307 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  MW400_RS23635 (MW400_23625) nikA 5005418..5006992 (+) 1575 WP_000493127.1 nickel ABC transporter substrate-binding protein -
  MW400_RS23640 (MW400_23630) nikB 5006992..5007936 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  MW400_RS23645 (MW400_23635) nikC 5007933..5008766 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  MW400_RS23650 (MW400_23640) amiE 5008766..5009530 (+) 765 WP_001136218.1 nickel import ATP-binding protein NikD Regulator
  MW400_RS23655 (MW400_23645) nikE 5009527..5010333 (+) 807 WP_000173692.1 nickel import ATP-binding protein NikE -
  MW400_RS23660 (MW400_23650) nikR 5010339..5010740 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26782.38 Da        Isoelectric Point: 6.6882

>NTDB_id=583883 MW400_RS23650 WP_001136218.1 5008766..5009530(+) (amiE) [Escherichia coli strain GN02461]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=583883 MW400_RS23650 WP_001136218.1 5008766..5009530(+) (amiE) [Escherichia coli strain GN02461]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCCGCACTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAAGCACGCATCCTCGATCTACTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCGCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398