Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   MW397_RS21610 Genome accession   NZ_CP095529
Coordinates   4512151..4512915 (+) Length   254 a.a.
NCBI ID   WP_029305321.1    Uniprot ID   -
Organism   Escherichia coli strain GN06156     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4507151..4517915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW397_RS21590 (MW397_21580) acpT 4508105..4508692 (+) 588 WP_000285765.1 4'-phosphopantetheinyl transferase AcpT -
  MW397_RS21595 (MW397_21585) nikA 4508803..4510377 (+) 1575 WP_021564688.1 nickel ABC transporter substrate-binding protein -
  MW397_RS21600 (MW397_21590) nikB 4510377..4511321 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  MW397_RS21605 (MW397_21595) nikC 4511318..4512151 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  MW397_RS21610 (MW397_21600) amiE 4512151..4512915 (+) 765 WP_029305321.1 nickel import ATP-binding protein NikD Regulator
  MW397_RS21615 (MW397_21605) nikE 4512912..4513718 (+) 807 WP_021564690.1 nickel import ATP-binding protein NikE -
  MW397_RS21620 (MW397_21610) nikR 4513724..4514125 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  MW397_RS21625 (MW397_21615) - 4514324..4515070 (+) 747 WP_021564691.1 GntR family transcriptional regulator -
  MW397_RS21630 (MW397_21620) - 4515095..4515568 (+) 474 WP_001306344.1 PTS sugar transporter subunit IIA -
  MW397_RS21635 (MW397_21625) - 4515565..4515846 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  MW397_RS21640 (MW397_21630) - 4515923..4517281 (+) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26842.47 Da        Isoelectric Point: 6.9016

>NTDB_id=583792 MW397_RS21610 WP_029305321.1 4512151..4512915(+) (amiE) [Escherichia coli strain GN06156]
MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADNATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=583792 MW397_RS21610 WP_029305321.1 4512151..4512915(+) (amiE) [Escherichia coli strain GN06156]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGTGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATAACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402