Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   MW401_RS02535 Genome accession   NZ_CP095510
Coordinates   546409..547173 (-) Length   254 a.a.
NCBI ID   WP_001136250.1    Uniprot ID   -
Organism   Escherichia coli strain GN02270     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 541409..552173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW401_RS02505 (MW401_02505) - 542044..543402 (-) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -
  MW401_RS02510 (MW401_02510) - 543479..543760 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  MW401_RS02515 (MW401_02515) - 543757..544230 (-) 474 WP_001377099.1 PTS sugar transporter subunit IIA -
  MW401_RS02520 (MW401_02520) - 544255..545001 (-) 747 WP_001322918.1 GntR family transcriptional regulator -
  MW401_RS02525 (MW401_02525) nikR 545200..545601 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  MW401_RS02530 (MW401_02530) nikE 545606..546412 (-) 807 WP_000173693.1 nickel import ATP-binding protein NikE -
  MW401_RS02535 (MW401_02535) amiE 546409..547173 (-) 765 WP_001136250.1 nickel import ATP-binding protein NikD Regulator
  MW401_RS02540 (MW401_02540) nikC 547173..548006 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  MW401_RS02545 (MW401_02545) nikB 548003..548947 (-) 945 WP_000947088.1 nickel ABC transporter permease subunit NikB -
  MW401_RS02550 (MW401_02550) nikA 548947..550521 (-) 1575 WP_000493127.1 nickel ABC transporter substrate-binding protein -
  MW401_RS02555 (MW401_02555) acpT 550632..551219 (-) 588 WP_000285765.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=583311 MW401_RS02535 WP_001136250.1 546409..547173(-) (amiE) [Escherichia coli strain GN02270]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGSSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAALEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=583311 MW401_RS02535 WP_001136250.1 546409..547173(-) (amiE) [Escherichia coli strain GN02270]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAACCGCTGGTGCACGGCGTATCGTTAACTCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCAGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCCGCACTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTTCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCTTTAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398