Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   MW398_RS12760 Genome accession   NZ_CP095505
Coordinates   2366819..2367583 (+) Length   254 a.a.
NCBI ID   WP_021524585.1    Uniprot ID   -
Organism   Escherichia coli strain GN02050     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2361819..2372583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW398_RS12740 (MW398_12730) acpT 2362773..2363360 (+) 588 WP_000285790.1 4'-phosphopantetheinyl transferase AcpT -
  MW398_RS12745 (MW398_12735) nikA 2363471..2365045 (+) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  MW398_RS12750 (MW398_12740) nikB 2365045..2365989 (+) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  MW398_RS12755 (MW398_12745) nikC 2365986..2366819 (+) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  MW398_RS12760 (MW398_12750) amiE 2366819..2367583 (+) 765 WP_021524585.1 nickel import ATP-binding protein NikD Regulator
  MW398_RS12765 (MW398_12755) nikE 2367580..2368386 (+) 807 WP_021524586.1 nickel import ATP-binding protein NikE -
  MW398_RS12770 (MW398_12760) nikR 2368392..2368793 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  MW398_RS12775 (MW398_12765) yhhJ 2368802..2369926 (-) 1125 WP_059342396.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26854.48 Da        Isoelectric Point: 6.6882

>NTDB_id=583248 MW398_RS12760 WP_021524585.1 2366819..2367583(+) (amiE) [Escherichia coli strain GN02050]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGIRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNVPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=583248 MW398_RS12760 WP_021524585.1 2366819..2367583(+) (amiE) [Escherichia coli strain GN02050]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCATTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGACGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGTCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398