Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   MWN28_RS01475 Genome accession   NZ_CP095448
Coordinates   332962..333726 (-) Length   254 a.a.
NCBI ID   WP_001136211.1    Uniprot ID   -
Organism   Escherichia coli strain LS45     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 327962..338726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWN28_RS01445 (MWN28_01445) - 328599..329957 (-) 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
  MWN28_RS01450 (MWN28_01450) - 330031..330312 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  MWN28_RS01455 (MWN28_01455) - 330309..330782 (-) 474 WP_012311717.1 PTS sugar transporter subunit IIA -
  MWN28_RS01460 (MWN28_01460) - 330807..331553 (-) 747 WP_001329803.1 GntR family transcriptional regulator -
  MWN28_RS01465 (MWN28_01465) nikR 331752..332153 (-) 402 WP_001189000.1 nickel-responsive transcriptional regulator NikR -
  MWN28_RS01470 (MWN28_01470) nikE 332159..332965 (-) 807 WP_000173687.1 nickel import ATP-binding protein NikE -
  MWN28_RS01475 (MWN28_01475) amiE 332962..333726 (-) 765 WP_001136211.1 nickel import ATP-binding protein NikD Regulator
  MWN28_RS01480 (MWN28_01480) nikC 333726..334559 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  MWN28_RS01485 (MWN28_01485) nikB 334556..335498 (-) 943 Protein_294 nickel ABC transporter permease subunit NikB -
  MWN28_RS01490 (MWN28_01490) nikA 335498..337072 (-) 1575 WP_000493128.1 nickel ABC transporter substrate-binding protein -
  MWN28_RS01495 (MWN28_01495) acpT 337183..337770 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.45 Da        Isoelectric Point: 6.9016

>NTDB_id=582893 MWN28_RS01475 WP_001136211.1 332962..333726(-) (amiE) [Escherichia coli strain LS45]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=582893 MWN28_RS01475 WP_001136211.1 332962..333726(-) (amiE) [Escherichia coli strain LS45]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTTCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTTGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATTCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398