Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   MU524_RS01190 Genome accession   NZ_CP095271
Coordinates   261129..261893 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain EC9682     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 256129..266893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU524_RS01160 (MU524_01160) - 256664..257848 (-) 1185 Protein_230 PTS galactitol transporter subunit IIC -
  MU524_RS01165 (MU524_01165) - 257906..258603 (+) 698 WP_223230114.1 IS1-like element IS1A family transposase -
  MU524_RS01170 (MU524_01170) - 258623..258949 (-) 327 Protein_232 PTS sugar transporter subunit IIA -
  MU524_RS01175 (MU524_01175) - 258974..259720 (-) 747 WP_001332161.1 GntR family transcriptional regulator -
  MU524_RS01180 (MU524_01180) nikR 259919..260320 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  MU524_RS01185 (MU524_01185) nikE 260326..261132 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  MU524_RS01190 (MU524_01190) amiE 261129..261893 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  MU524_RS01195 (MU524_01195) nikC 261893..262726 (-) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  MU524_RS01200 (MU524_01200) nikB 262723..263667 (-) 945 WP_000947074.1 nickel ABC transporter permease subunit NikB -
  MU524_RS01205 (MU524_01205) nikA 263667..265241 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  MU524_RS01210 (MU524_01210) acpT 265352..265939 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=582808 MU524_RS01190 WP_001136232.1 261129..261893(-) (amiE) [Escherichia coli strain EC9682]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=582808 MU524_RS01190 WP_001136232.1 261129..261893(-) (amiE) [Escherichia coli strain EC9682]
ATGCCACAACAGATTGAGCTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGAATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCA
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398