Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   MU416_RS01210 Genome accession   NZ_CP095125
Coordinates   265476..266240 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain Iso00270     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 260476..271240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU416_RS01200 nikR 264266..264667 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  MU416_RS01205 nikE 264673..265479 (-) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  MU416_RS01210 amiE 265476..266240 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  MU416_RS01215 nikC 266240..267073 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  MU416_RS01220 nikB 267070..268014 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  MU416_RS01225 nikA 268014..269588 (-) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  MU416_RS01230 acpT 269699..270286 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=582401 MU416_RS01210 WP_001136229.1 265476..266240(-) (amiE) [Escherichia coli strain Iso00270]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=582401 MU416_RS01210 WP_001136229.1 265476..266240(-) (amiE) [Escherichia coli strain Iso00270]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398