Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   KP952_RS12565 Genome accession   NZ_CP076731
Coordinates   2361725..2362762 (-) Length   345 a.a.
NCBI ID   WP_031600685.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain Miz-8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2356725..2367762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KP952_RS12515 (KP952_12515) tasA 2356823..2357608 (-) 786 WP_014480250.1 biofilm matrix protein TasA -
  KP952_RS12520 (KP952_12520) sipW 2357672..2358244 (-) 573 WP_003230181.1 signal peptidase I SipW -
  KP952_RS12525 (KP952_12525) tapA 2358228..2358989 (-) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -
  KP952_RS12530 (KP952_12530) yqzG 2359261..2359587 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  KP952_RS12535 (KP952_12535) spoIITA 2359629..2359808 (-) 180 WP_014480252.1 YqzE family protein -
  KP952_RS12540 (KP952_12540) comGG 2359879..2360253 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  KP952_RS12545 (KP952_12545) comGF 2360254..2360637 (-) 384 WP_029317913.1 ComG operon protein ComGF Machinery gene
  KP952_RS12550 (KP952_12550) comGE 2360663..2361010 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  KP952_RS12555 (KP952_12555) comGD 2360994..2361425 (-) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  KP952_RS12560 (KP952_12560) comGC 2361415..2361711 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  KP952_RS12565 (KP952_12565) comGB 2361725..2362762 (-) 1038 WP_031600685.1 comG operon protein ComGB Machinery gene
  KP952_RS12570 (KP952_12570) comGA 2362749..2363819 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  KP952_RS12575 (KP952_12575) - 2364031..2364228 (-) 198 WP_014480259.1 CBS domain-containing protein -
  KP952_RS12580 (KP952_12580) corA 2364230..2365183 (-) 954 WP_014480260.1 magnesium transporter CorA -
  KP952_RS12585 (KP952_12585) yqhB 2365325..2366653 (+) 1329 WP_014480261.1 hemolysin family protein -
  KP952_RS12590 (KP952_12590) rsbRD 2366706..2367542 (-) 837 WP_014480262.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39592.99 Da        Isoelectric Point: 9.6259

>NTDB_id=577767 KP952_RS12565 WP_031600685.1 2361725..2362762(-) (comGB) [Bacillus subtilis subsp. subtilis strain Miz-8]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTGSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKSVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=577767 KP952_RS12565 WP_031600685.1 2361725..2362762(-) (comGB) [Bacillus subtilis subsp. subtilis strain Miz-8]
ATGAAAAAGATTAGAAAAGTCTGGTCGTTAAAGGATCAAGCGAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGGTTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGTGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.071

93.623

0.928