Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   KPL75_RS08745 Genome accession   NZ_CP076653
Coordinates   1656758..1659079 (-) Length   773 a.a.
NCBI ID   WP_219920374.1    Uniprot ID   -
Organism   Bacillus sp. NP247     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1651758..1664079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KPL75_RS08715 (KPL75_08710) - 1652194..1652370 (-) 177 WP_001242605.1 hypothetical protein -
  KPL75_RS08720 (KPL75_08715) - 1652455..1652646 (-) 192 WP_219920372.1 damage-inducible protein DinB -
  KPL75_RS08725 (KPL75_08720) - 1652726..1653058 (-) 333 WP_219920373.1 YrdB family protein -
  KPL75_RS08730 (KPL75_08725) - 1653326..1653766 (-) 441 WP_046197788.1 D-tyrosyl-tRNA(Tyr) deacylase -
  KPL75_RS08735 (KPL75_08730) relA 1653784..1655967 (-) 2184 WP_002145623.1 GTP diphosphokinase -
  KPL75_RS08740 (KPL75_08735) - 1656180..1656692 (-) 513 WP_000346222.1 adenine phosphoribosyltransferase -
  KPL75_RS08745 (KPL75_08740) recJ 1656758..1659079 (-) 2322 WP_219920374.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  KPL75_RS08750 (KPL75_08745) - 1659181..1660074 (-) 894 WP_219920375.1 cation diffusion facilitator family transporter -
  KPL75_RS08755 (KPL75_08750) secDF 1660229..1662493 (-) 2265 WP_219920376.1 protein translocase subunit SecDF -
  KPL75_RS08760 (KPL75_08755) - 1662775..1663059 (-) 285 WP_002112000.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 773 a.a.        Molecular weight: 87305.87 Da        Isoelectric Point: 5.1185

>NTDB_id=576989 KPL75_RS08745 WP_219920374.1 1656758..1659079(-) (recJ) [Bacillus sp. NP247]
MLQPKTRWKEKKYNEELVSELANKLQLSPLVTSLFLGRGLNTEDKIVDFLNIADQEFHDPFLLEGMDRTVERVKKAIENG
EQILIFGDYDADGVSSTTVLYLALKELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VQRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSLAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENKFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPHKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLNMNDVDELRRRLNEQADVILTAEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDADLESIRAIGSDGSHLKMALRDGQATLDTIGFGFGTYAKEISPVAKVSVVGEVSINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKEKITMVYFSEEVLNKFSVDGYKEQLVHASEVAHFENQYIVLLDLPKGTDELGD
VFKTGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHK

Nucleotide


Download         Length: 2322 bp        

>NTDB_id=576989 KPL75_RS08745 WP_219920374.1 1656758..1659079(-) (recJ) [Bacillus sp. NP247]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAAAATATAATGAGGAACTAGTGAGTGAATTAGCAAATAAATTGCAACT
ATCACCTCTTGTGACTTCATTATTTCTCGGCAGAGGCTTAAATACAGAAGATAAGATTGTAGACTTTTTAAATATAGCAG
ATCAAGAATTTCATGATCCATTTTTACTTGAAGGAATGGATCGGACTGTAGAACGTGTGAAAAAAGCTATTGAAAATGGA
GAACAAATATTAATATTTGGCGATTATGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCTCTCAAAGAATT
AGGTGCAGAAGTAGAGTTTTATATTCCAAATCGTTTTACTGAAGGTTATGGACCAAATGAAGAAGCGTTTCGTTGGGCGC
ACAGTGCAGGGTTCTCACTAATTATCACTGTCGATACTGGTATCGCAGCGGTACATGAAGCGAAGGTAGCGAAGGAACTT
GGAATAGATCTTATTATTACAGATCATCATGAGCCACCACCAGAATTACCAGAGGCACTTGCCATTATTCATCCGAAATT
AGAAGGCGGTGTTTATCCGTTTCATTATTTAGCGGGTGTTGGTGTTGCATTTAAAGTTGCACATGCACTTTTGGGCCGTG
TACCAGAGCATTTGTTGGAAATTGCAGTAATCGGTACGGTAGCCGATTTAGTATCGCTTCACGGTGAAAATAGATTGTTA
GTACAGCGTGGACTGAAGCATATGCGAATGACTCGAAATATCGGTTTAAAGGCGTTATTTAAAGTTGCTAACGTTTCGCA
AAGCGAAATTACAGAAGAAAGCATCGGATTCTCACTCGCACCTCGTATTAATGCAGTTGGTCGTTTGGAGGATGCAACAC
CTGCTGTACACCTTTTATTATCAGAAGATCCAGAGGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGCTGAACAAACTT
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTAGAAAATAAATTCCCGCCGGAAGAAAATAAAGT
ATTAGTCCTTGCAAAAGAAGGTTGGAATCCAGGGGTAATTGGAATTGTCGCTTCTAAGTTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATTGATCCTCATAAAGAAACAGCAAAGGGATCGGCTCGTAGTATTGCAGGATTTGATTTGTTT
GCAAACCTTTCAGATTGCCGTGAATTGTTACCGCATTTCGGAGGTCATCCGATGGCGGCGGGAATGACGCTTAATATGAA
TGATGTGGATGAATTAAGACGCAGGTTAAATGAGCAAGCAGATGTAATTTTAACAGCAGAAGATTTTATTCCGATTACAG
CAGTGGATGCATTTTGTAAGGTAGAGGATGTAACGCTTGCCGCGATTGAGGATATGCAAAAGTTAGCGCCATTTGGCGTA
GGAAATCCGAAACCACGTATTGCGGTGAAAGATGCTGACTTAGAAAGTATCCGTGCAATTGGATCTGATGGTTCGCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGACACGATTGGATTTGGATTTGGTACTTATGCGAAAGAAATCTCTC
CAGTTGCGAAGGTTTCTGTAGTAGGAGAAGTGTCCATTAATGAATGGAATAACTTTAAAAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGACTGGCGTAGTATGCGTAATGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACCATGGTGTATTTTTCTGAAGAGGTATTGAATAAGTTTTCTGTAGACGGCTATAAAGAACAGCTCGTGC
ATGCATCAGAGGTAGCTCATTTTGAGAATCAATACATTGTGCTACTTGATTTGCCGAAAGGAACAGATGAATTAGGTGAT
GTATTTAAGACTGGATTCCCAGCTCGAATTTACACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACGGTCCCAAC
GAGGGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGGCAATATGGGGAACAACTGT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCCGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAT
AA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.258

100

0.524