Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   KOM03_RS16750 Genome accession   NZ_CP076450
Coordinates   3452993..3453793 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain GMEKP1     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 3447993..3458793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOM03_RS16720 (KOM03_16720) - 3448531..3449163 (+) 633 WP_014418348.1 helix-turn-helix domain-containing protein -
  KOM03_RS16725 (KOM03_16725) - 3449460..3449597 (+) 138 WP_014470646.1 hypothetical protein -
  KOM03_RS16730 (KOM03_16730) - 3449691..3450809 (+) 1119 WP_032863662.1 NADH-dependent flavin oxidoreductase -
  KOM03_RS16735 (KOM03_16735) - 3450936..3451148 (+) 213 WP_007408362.1 spore germination protein -
  KOM03_RS16740 (KOM03_16740) - 3451274..3451486 (+) 213 WP_003153181.1 spore germination protein -
  KOM03_RS16745 (KOM03_16745) alr 3451663..3452844 (+) 1182 WP_060562602.1 alanine racemase -
  KOM03_RS16750 (KOM03_16750) spo0A 3452993..3453793 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  KOM03_RS16755 (KOM03_16755) spoIVB 3454074..3455354 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  KOM03_RS16760 (KOM03_16760) recN 3455518..3457247 (-) 1730 Protein_3274 DNA repair protein RecN -
  KOM03_RS16765 (KOM03_16765) argR 3457279..3457728 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  KOM03_RS16770 (KOM03_16770) - 3457825..3458670 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=575489 KOM03_RS16750 WP_003153177.1 3452993..3453793(-) (spo0A) [Bacillus velezensis strain GMEKP1]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=575489 KOM03_RS16750 WP_003153177.1 3452993..3453793(-) (spo0A) [Bacillus velezensis strain GMEKP1]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATTGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCTGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCACAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97