Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   KOM03_RS03365 Genome accession   NZ_CP076450
Coordinates   657397..657516 (+) Length   39 a.a.
NCBI ID   WP_015240778.1    Uniprot ID   -
Organism   Bacillus velezensis strain GMEKP1     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 652397..662516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOM03_RS03345 (KOM03_03345) - 652747..653259 (+) 513 Protein_653 bacteriocin biosynthesis protein AlbG -
  KOM03_RS03350 (KOM03_03350) - 653266..654642 (-) 1377 WP_060562419.1 YncE family protein -
  KOM03_RS03355 (KOM03_03355) - 654682..656037 (-) 1356 WP_060562418.1 YncE family protein -
  KOM03_RS03360 (KOM03_03360) rapF 656268..657413 (+) 1146 WP_007407638.1 tetratricopeptide repeat protein Regulator
  KOM03_RS03365 (KOM03_03365) phrF 657397..657516 (+) 120 WP_015240778.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  KOM03_RS03370 (KOM03_03370) - 657714..657917 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  KOM03_RS03375 (KOM03_03375) - 657931..658134 (+) 204 WP_007407641.1 hypothetical protein -
  KOM03_RS03380 (KOM03_03380) - 658169..658354 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  KOM03_RS03385 (KOM03_03385) - 658541..658822 (+) 282 WP_007407643.1 hypothetical protein -
  KOM03_RS03390 (KOM03_03390) - 658866..659378 (+) 513 WP_031378560.1 stage II sporulation protein M -
  KOM03_RS03395 (KOM03_03395) - 659969..660187 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  KOM03_RS03400 (KOM03_03400) speB 660353..661225 (-) 873 WP_007614448.1 agmatinase -
  KOM03_RS03405 (KOM03_03405) speE 661284..662114 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4281.16 Da        Isoelectric Point: 10.0233

>NTDB_id=575448 KOM03_RS03365 WP_015240778.1 657397..657516(+) (phrF) [Bacillus velezensis strain GMEKP1]
MKLKYKLALTCLAISTVFVSADIASAPKHQFEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=575448 KOM03_RS03365 WP_015240778.1 657397..657516(+) (phrF) [Bacillus velezensis strain GMEKP1]
ATGAAATTGAAGTATAAATTAGCACTTACTTGCTTGGCAATAAGCACTGTTTTTGTTTCAGCCGACATCGCAAGTGCACC
GAAACATCAATTTGAAGTTGCTCAGCGCGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

74.074

69.231

0.513
  phrC Bacillus subtilis subsp. subtilis str. 168

46.154

100

0.462