Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KHF85_RS06220 Genome accession   NZ_CP076255
Coordinates   1442564..1443694 (+) Length   376 a.a.
NCBI ID   WP_284568919.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. graminis strain NCPPB 3711     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1437564..1448694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHF85_RS06195 (KHF85_06180) - 1437624..1438079 (-) 456 WP_284568916.1 DUF4426 domain-containing protein -
  KHF85_RS06200 (KHF85_06185) proC 1438094..1438936 (-) 843 WP_284568917.1 pyrroline-5-carboxylate reductase -
  KHF85_RS06205 (KHF85_06190) - 1439066..1440529 (-) 1464 WP_284568918.1 hypothetical protein -
  KHF85_RS06210 (KHF85_06195) - 1440603..1441253 (-) 651 WP_284569486.1 YggS family pyridoxal phosphate-dependent enzyme -
  KHF85_RS06215 (KHF85_06200) pilT 1441378..1442415 (+) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  KHF85_RS06220 (KHF85_06205) pilU 1442564..1443694 (+) 1131 WP_284568919.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KHF85_RS06225 (KHF85_06210) - 1444111..1444650 (-) 540 WP_284568920.1 DNA-3-methyladenine glycosylase I -
  KHF85_RS06230 (KHF85_06215) - 1444666..1446474 (-) 1809 WP_284568921.1 DUF4153 domain-containing protein -
  KHF85_RS06235 (KHF85_06220) - 1446582..1447148 (+) 567 WP_009599956.1 YqgE/AlgH family protein -
  KHF85_RS06240 (KHF85_06225) ruvX 1447141..1447638 (+) 498 WP_284568922.1 Holliday junction resolvase RuvX -
  KHF85_RS06245 (KHF85_06230) - 1447658..1448605 (+) 948 WP_003478088.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41940.26 Da        Isoelectric Point: 6.8811

>NTDB_id=574096 KHF85_RS06220 WP_284568919.1 1442564..1443694(+) (pilU) [Xanthomonas translucens pv. graminis strain NCPPB 3711]
MSTIDFTSFLKLMAHQKASDLFITAGMPPAIKVHGKITPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNMRGVVAQQLIPTPDGKSRRVAIEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=574096 KHF85_RS06220 WP_284568919.1 1442564..1443694(+) (pilU) [Xanthomonas translucens pv. graminis strain NCPPB 3711]
ATGAGCACCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCGCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
CACCGTCGATGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCTGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCTCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAATGCCAATCAGGCGATG
GACCGGATCATCAACTTCTTTCCCGAAGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAACATGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGAGCCGCCGCGTGGCGATCGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGTTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACCCTGGCGCAGGGCATGGACGGGGTGGAAATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.854

98.138

0.646
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367