Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   MMZ75_RS13270 Genome accession   NZ_CP093016
Coordinates   2863391..2863816 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain H15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2858391..2868816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ75_RS13250 (MMZ75_13245) ileS 2858548..2861379 (+) 2832 WP_003123398.1 isoleucine--tRNA ligase -
  MMZ75_RS13255 (MMZ75_13250) lspA 2861372..2861881 (+) 510 WP_003102615.1 signal peptidase II -
  MMZ75_RS13260 (MMZ75_13255) fkpB 2861874..2862314 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  MMZ75_RS13265 (MMZ75_13260) ispH 2862400..2863344 (+) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  MMZ75_RS13270 (MMZ75_13265) comF 2863391..2863816 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  MMZ75_RS13275 (MMZ75_13270) pilY2 2863813..2864160 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  MMZ75_RS13280 (MMZ75_13275) pilY1 2864162..2867653 (-) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  MMZ75_RS13285 (MMZ75_13280) pilX 2867665..2868252 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=569472 MMZ75_RS13270 WP_003094721.1 2863391..2863816(-) (comF) [Pseudomonas aeruginosa strain H15]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=569472 MMZ75_RS13270 WP_003094721.1 2863391..2863816(-) (comF) [Pseudomonas aeruginosa strain H15]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383