Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   MKF35_RS12245 Genome accession   NZ_CP092629
Coordinates   2650747..2651172 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain HU20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2645747..2656172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS12225 ileS 2645904..2648735 (+) 2832 WP_003102617.1 isoleucine--tRNA ligase -
  MKF35_RS12230 lspA 2648728..2649237 (+) 510 WP_003102615.1 signal peptidase II -
  MKF35_RS12235 fkpB 2649230..2649670 (+) 441 WP_043556111.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  MKF35_RS12240 ispH 2649756..2650700 (+) 945 WP_003112824.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  MKF35_RS12245 comF 2650747..2651172 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  MKF35_RS12250 pilY2 2651169..2651516 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  MKF35_RS12255 pilY1 2651518..2655009 (-) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  MKF35_RS12260 pilX 2655021..2655608 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=567228 MKF35_RS12245 WP_003094721.1 2650747..2651172(-) (comF) [Pseudomonas aeruginosa strain HU20]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=567228 MKF35_RS12245 WP_003094721.1 2650747..2651172(-) (comF) [Pseudomonas aeruginosa strain HU20]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383