Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   KHA81_RS13140 Genome accession   NZ_CP074391
Coordinates   2695034..2697394 (-) Length   786 a.a.
NCBI ID   WP_015387899.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain L-17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2650991..2697470 2695034..2697394 within 0


Gene organization within MGE regions


Location: 2650991..2697470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHA81_RS12870 - 2651379..2651684 (-) 306 WP_015387929.1 hypothetical protein -
  KHA81_RS12875 - 2651684..2652283 (-) 600 WP_015387928.1 dUTP diphosphatase -
  KHA81_RS12880 - 2652280..2652603 (-) 324 WP_015387927.1 hypothetical protein -
  KHA81_RS12885 - 2652713..2653537 (-) 825 WP_015387925.1 DNA adenine methylase -
  KHA81_RS12890 - 2653551..2654927 (-) 1377 WP_015387924.1 DNA cytosine methyltransferase -
  KHA81_RS12895 - 2654932..2655189 (-) 258 WP_015387923.1 hypothetical protein -
  KHA81_RS12900 - 2655202..2655603 (-) 402 WP_015387922.1 hypothetical protein -
  KHA81_RS12905 - 2655600..2655830 (-) 231 WP_015387921.1 hypothetical protein -
  KHA81_RS12910 - 2655827..2656210 (-) 384 WP_015387920.1 hypothetical protein -
  KHA81_RS12915 - 2656351..2656554 (-) 204 WP_014721584.1 XtrA/YqaO family protein -
  KHA81_RS12920 - 2656634..2656789 (-) 156 WP_015387919.1 hypothetical protein -
  KHA81_RS12925 - 2656802..2656942 (-) 141 WP_015387918.1 BH0509 family protein -
  KHA81_RS12930 - 2657050..2657598 (-) 549 WP_015387916.1 hypothetical protein -
  KHA81_RS12935 - 2657595..2657753 (-) 159 WP_015387915.1 hypothetical protein -
  KHA81_RS12940 - 2657750..2657899 (-) 150 WP_015387914.1 hypothetical protein -
  KHA81_RS12945 - 2657914..2658747 (-) 834 WP_031306578.1 ATP-binding protein -
  KHA81_RS12950 - 2658731..2659603 (-) 873 WP_015387912.1 conserved phage C-terminal domain-containing protein -
  KHA81_RS12955 - 2659617..2659934 (-) 318 WP_015387911.1 hypothetical protein -
  KHA81_RS12960 - 2659975..2660166 (-) 192 WP_015387910.1 helix-turn-helix transcriptional regulator -
  KHA81_RS12965 - 2660336..2660725 (+) 390 WP_015387909.1 helix-turn-helix transcriptional regulator -
  KHA81_RS12970 - 2661096..2662277 (-) 1182 WP_014418511.1 AimR family lysis-lysogeny pheromone receptor -
  KHA81_RS12975 - 2662584..2663744 (+) 1161 WP_015387907.1 site-specific integrase -
  KHA81_RS12980 udk 2663809..2664441 (-) 633 WP_014418513.1 uridine kinase -
  KHA81_RS12985 - 2664448..2665716 (-) 1269 WP_014305485.1 U32 family peptidase -
  KHA81_RS12990 - 2665734..2666663 (-) 930 WP_003152757.1 peptidase U32 family protein -
  KHA81_RS12995 - 2666670..2667323 (-) 654 WP_015387906.1 O-methyltransferase -
  KHA81_RS13000 mltG 2667475..2668566 (-) 1092 WP_014305487.1 endolytic transglycosylase MltG -
  KHA81_RS13005 - 2668677..2668958 (-) 282 WP_003152751.1 DUF1292 domain-containing protein -
  KHA81_RS13010 ruvX 2668971..2669387 (-) 417 WP_003152750.1 Holliday junction resolvase RuvX -
  KHA81_RS13015 - 2669396..2669662 (-) 267 WP_003152749.1 IreB family regulatory phosphoprotein -
  KHA81_RS13020 alaS 2669747..2672383 (-) 2637 WP_003152748.1 alanine--tRNA ligase -
  KHA81_RS13025 - 2672715..2673776 (-) 1062 WP_015387905.1 AI-2E family transporter -
  KHA81_RS13030 - 2673996..2674724 (+) 729 WP_003152746.1 amino acid ABC transporter ATP-binding protein -
  KHA81_RS13035 - 2674745..2675572 (+) 828 WP_003152745.1 transporter substrate-binding domain-containing protein -
  KHA81_RS13040 - 2675629..2676279 (+) 651 WP_003152744.1 amino acid ABC transporter permease -
  KHA81_RS13045 - 2676297..2676953 (+) 657 WP_015387904.1 amino acid ABC transporter permease -
  KHA81_RS13050 - 2676989..2677120 (-) 132 WP_003152741.1 YrzQ family protein -
  KHA81_RS13055 - 2677142..2677333 (-) 192 WP_003152738.1 hypothetical protein -
  KHA81_RS13060 - 2677346..2677804 (-) 459 WP_003152735.1 PRC-barrel domain-containing protein -
  KHA81_RS13065 recD2 2677923..2680301 (-) 2379 WP_014305491.1 ATP-dependent RecD-like DNA helicase Machinery gene
  KHA81_RS13070 - 2680320..2680940 (-) 621 WP_088005508.1 tetratricopeptide repeat protein -
  KHA81_RS13075 mnmA 2681028..2682143 (-) 1116 WP_015387903.1 tRNA 2-thiouridine(34) synthase MnmA -
  KHA81_RS13080 - 2682180..2683319 (-) 1140 WP_003152731.1 cysteine desulfurase family protein -
  KHA81_RS13085 cymR 2683338..2683754 (-) 417 WP_003152730.1 cysteine metabolism transcriptional regulator CymR -
  KHA81_RS13090 - 2683949..2685217 (+) 1269 WP_015387902.1 replication-associated recombination protein A -
  KHA81_RS13095 - 2685293..2686057 (-) 765 WP_003152728.1 tRNA threonylcarbamoyladenosine dehydratase -
  KHA81_RS13105 aspS 2686384..2688162 (-) 1779 WP_015387901.1 aspartate--tRNA ligase -
  KHA81_RS13110 hisS 2688177..2689451 (-) 1275 WP_003152726.1 histidine--tRNA ligase -
  KHA81_RS13115 - 2689808..2689978 (-) 171 WP_003152722.1 YrzK family protein -
  KHA81_RS13120 - 2690109..2691671 (+) 1563 WP_015387900.1 SH3 domain-containing protein -
  KHA81_RS13125 dtd 2691698..2692141 (-) 444 WP_007408194.1 D-aminoacyl-tRNA deacylase -
  KHA81_RS13130 relA 2692154..2694358 (-) 2205 WP_003152716.1 GTP diphosphokinase -
  KHA81_RS13135 - 2694516..2695028 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  KHA81_RS13140 recJ 2695034..2697394 (-) 2361 WP_015387899.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87396.76 Da        Isoelectric Point: 4.9218

>NTDB_id=564571 KHA81_RS13140 WP_015387899.1 2695034..2697394(-) (recJ) [Bacillus amyloliquefaciens strain L-17]
MLASKMRWEIQQPDQEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKLAVSQG
EPIMIYGDYDADGVTSTSVMLTALRQLSADVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCGYPFKELAGVGVAFKLAHALTGSLPEAMLDLAAIGTVADLVPLHDENRLI
ASLGIERLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTEDQFEAEELASDIDRLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVAKAGWNPGVVGIVASKLAERFYRPAVVLGIDEEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVAELRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVESITELNMLSPFGMLN
PKPHVMVKNAVLDDVRLIGANKNHVKMTVKSDSAVLDCVGFNKGELEAGVVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVTALPKEKRVLISFNEKTAQKLREKELTGELAEITSESEAEALQLDGKYAVFLDVPPSADR
LQHLLDGKKPERIYFVFINHEEHFLSSFPSRNDFKWFYAFLLKRGAFDFGKHGSELARHRGWSIDTINFMTKVFFDLGFV
KIEDGVLSVVSGAKKRDLADSQTYQVKQQLMELDQKLNYSAAHELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=564571 KHA81_RS13140 WP_015387899.1 2695034..2697394(-) (recJ) [Bacillus amyloliquefaciens strain L-17]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATCAGGAAAAAGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCACTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAACTGGCGGTTTCTCAAGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCAGTGATGCTGACGGCACTCAGACAGCT
GTCGGCGGACGTTGACTTTTATATTCCCGACCGTTTTACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGTTCCATAA
AAGAGCGGGGATTTTCGCTGATCATTACAGTGGATACGGGAATCGCCGCCGTCCATGAAGCTGAAGTCGCAAAAGAGCTG
GGCTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATCATCCACCCGAAGCA
GCCGGGATGCGGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTGGCGTTTAAGCTTGCCCACGCGCTTACGGGTTCTT
TGCCGGAAGCTATGTTGGACTTGGCGGCAATCGGTACGGTTGCAGATCTGGTGCCTCTTCATGATGAAAACAGATTAATT
GCGTCACTCGGAATTGAAAGGCTTCGTACGACAAATCGGCCGGGCCTCAAAGCTCTGATTAAACTTTCCGGCGGAGATAT
CGGACAAGCTGATGAGGAAACGATCGGATTTCAGGTCGCACCGAGGCTCAATGCCGTCGGAAGAATAGAACAGGCCGATC
CTGCCGTACACCTGCTGATGACTGAAGATCAGTTTGAAGCGGAGGAACTGGCTTCGGATATTGACCGGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTTCAGCAGCAAGGCGGAAACCCGTCTGCCATTGT
AGTGGCAAAAGCCGGCTGGAATCCGGGCGTCGTCGGTATTGTCGCATCGAAGCTGGCTGAACGTTTTTACCGGCCCGCTG
TTGTGCTTGGGATAGATGAGGAGCAGGGAATCGCCAAAGGGTCGGCGCGAAGCATCAGAGGCTTCAACCTTTTTACGAGC
CTTTCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGGCATCCGATGGCGGCCGGAATGACGCTGAAGGCTGAAGATGT
GGCGGAATTAAGGGACAGGCTGAATCAAATGGCTGAAGAAACCCTTACGGAAGAGGATTTTATTCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTTGAAGACATAACCGTTGAAAGCATTACAGAATTGAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCGCATGTGATGGTGAAAAATGCCGTGCTTGATGATGTGCGCCTGATCGGAGCGAATAAAAACCACGTGAAAAT
GACGGTGAAAAGTGATTCCGCCGTCCTTGACTGCGTCGGCTTTAACAAAGGAGAGCTGGAAGCCGGTGTTGTTCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCCATTAATGAATGGAACAACAGAAAGAAGCCCCAGCTGATGATTAAAGATGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATCCGGGGCAAGCGGACGTGGGAAGATACGGTCACCGCGCTGCCGAAGGAGAA
ACGGGTGCTCATCAGCTTTAACGAAAAAACCGCTCAGAAGCTCCGTGAAAAAGAGCTGACGGGCGAGCTTGCCGAGATCA
CTTCGGAATCCGAGGCGGAAGCTCTCCAGCTTGACGGAAAATATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGATCGC
CTGCAGCATTTGCTTGACGGAAAGAAGCCGGAACGGATTTATTTTGTTTTTATCAATCATGAAGAACATTTCCTGTCCTC
ATTTCCATCGCGGAATGATTTCAAGTGGTTTTACGCATTTCTCTTGAAAAGGGGCGCTTTTGACTTCGGCAAGCATGGTT
CAGAGCTTGCGAGACACAGAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAAGATGGTGTGCTGTCTGTCGTGAGCGGTGCGAAAAAACGCGACCTGGCAGATTCGCAAACCTATCAGGTAAA
ACAGCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCAGCCCATGAGCTGAAGGAATGGCTGAATAAGCTGATGA
ACCAAGGTTCAGAGGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

82.061

100

0.821