Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MCS27_RS00725 Genome accession   NZ_CP092049
Coordinates   173631..174584 (-) Length   317 a.a.
NCBI ID   WP_004583795.1    Uniprot ID   Q7MXM2
Organism   Porphyromonas gingivalis strain W50/BE1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 168631..179584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCS27_RS00705 (MCS27_00705) lptE 169218..169757 (-) 540 WP_005875323.1 LPS assembly lipoprotein LptE -
  MCS27_RS00710 (MCS27_00710) - 169744..170997 (-) 1254 WP_004583792.1 sigma-54-dependent Fis family transcriptional regulator -
  MCS27_RS00715 (MCS27_00715) - 171025..172293 (-) 1269 WP_005875306.1 LysM peptidoglycan-binding domain-containing protein -
  MCS27_RS00720 (MCS27_00720) rimO 172330..173634 (-) 1305 WP_010955935.1 30S ribosomal protein S12 methylthiotransferase RimO -
  MCS27_RS00725 (MCS27_00725) pilA 173631..174584 (-) 954 WP_004583795.1 signal recognition particle-docking protein FtsY Machinery gene
  MCS27_RS00730 (MCS27_00730) nspC 174611..175747 (-) 1137 WP_005875334.1 carboxynorspermidine decarboxylase -
  MCS27_RS00735 (MCS27_00735) aspS 175757..177520 (-) 1764 WP_005875297.1 aspartate--tRNA ligase -
  MCS27_RS10335 - 177706..177954 (-) 249 WP_143733557.1 DUF1661 domain-containing protein -
  MCS27_RS00740 (MCS27_00740) ribD 177951..178944 (-) 994 Protein_142 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 34180.21 Da        Isoelectric Point: 5.8684

>NTDB_id=564551 MCS27_RS00725 WP_004583795.1 173631..174584(-) (pilA) [Porphyromonas gingivalis strain W50/BE1]
MGLFGLFSKKKKETLDEGLSKTKENVFAKITRAVAGKSKVDDEVLDELENVLVTSDVGVDTTLKIIKRIEERVARDKYVS
TSELTTILRQEIASLLTENGSADGDSFDLPSGTKPYVIMVVGVNGVGKTTTIGKLAHRFRQAGKSVVLGAADTFRAAAVE
QLEIWSERVGVPIIKQQMGSDPASVAYDTVSSAVANGVDVVIIDTAGRLHNKVNLMNELTKIKRVMQKVVPDAPHEILLV
LDGSTGQNAFEQARQFTAATEVNALAVTKLDGTAKGGVVIGISDQFKIPVKYIGLGEGIDDLQIFRKKEFVDSLFGE

Nucleotide


Download         Length: 954 bp        

>NTDB_id=564551 MCS27_RS00725 WP_004583795.1 173631..174584(-) (pilA) [Porphyromonas gingivalis strain W50/BE1]
ATGGGATTATTCGGATTATTCTCGAAAAAGAAAAAAGAGACTTTGGACGAAGGCCTGTCCAAGACCAAAGAAAACGTATT
CGCCAAGATCACCCGTGCCGTAGCCGGTAAGAGCAAAGTGGACGATGAGGTCTTGGACGAATTGGAAAACGTGCTGGTGA
CTTCGGATGTGGGTGTCGATACCACATTGAAGATCATCAAACGTATAGAGGAACGAGTGGCTCGCGACAAATATGTTTCC
ACCTCCGAGCTGACGACTATCCTCCGTCAAGAGATCGCCTCGCTGCTCACGGAGAACGGATCGGCCGATGGCGACAGCTT
CGACCTACCCTCCGGTACCAAACCTTATGTGATCATGGTAGTGGGCGTAAACGGCGTAGGCAAGACCACTACCATCGGCA
AGCTGGCACATCGCTTTCGTCAGGCCGGCAAGAGCGTCGTGCTCGGGGCTGCCGATACCTTCCGTGCTGCGGCCGTAGAG
CAGTTGGAGATCTGGTCCGAGCGAGTAGGAGTGCCTATCATCAAGCAGCAGATGGGTAGCGATCCGGCTTCGGTGGCTTA
TGACACAGTCAGTTCGGCCGTGGCTAACGGCGTCGATGTCGTGATCATCGATACAGCCGGACGTCTGCACAATAAGGTCA
ATCTGATGAACGAGCTGACGAAGATCAAGCGTGTGATGCAGAAGGTAGTGCCCGACGCTCCGCATGAGATTTTATTGGTA
TTGGACGGATCCACCGGTCAGAATGCTTTCGAACAGGCAAGGCAGTTCACCGCAGCGACTGAAGTGAATGCCCTCGCCGT
GACCAAGCTCGACGGGACAGCTAAGGGAGGTGTGGTGATCGGCATATCCGATCAGTTCAAGATACCGGTGAAGTACATTG
GCTTGGGTGAGGGCATAGACGACCTGCAGATCTTCCGTAAGAAGGAATTCGTGGACTCTCTCTTTGGCGAATGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(116-315)

SRP54-type protein, helical bundle domain (SRP54_N)

(29-91)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7MXM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

44.85

94.953

0.426