Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   KHA74_RS13685 Genome accession   NZ_CP074348
Coordinates   2800367..2802727 (-) Length   786 a.a.
NCBI ID   WP_094032217.1    Uniprot ID   -
Organism   Bacillus velezensis strain SW5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2795367..2807727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHA74_RS13665 (KHA74_13580) - 2795438..2797000 (+) 1563 WP_032866628.1 SH3 domain-containing protein -
  KHA74_RS13670 (KHA74_13585) dtd 2797029..2797472 (-) 444 WP_007408194.1 D-aminoacyl-tRNA deacylase -
  KHA74_RS13675 (KHA74_13590) relA 2797487..2799691 (-) 2205 WP_003152716.1 GTP diphosphokinase -
  KHA74_RS13680 (KHA74_13595) - 2799849..2800361 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  KHA74_RS13685 (KHA74_13600) recJ 2800367..2802727 (-) 2361 WP_094032217.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  KHA74_RS13690 (KHA74_13605) - 2802783..2803109 (-) 327 WP_072177620.1 lipopolysaccharide assembly LapA domain-containing protein -
  KHA74_RS13695 (KHA74_13610) - 2803173..2803670 (-) 498 WP_012118088.1 cation:proton antiporter regulatory subunit -
  KHA74_RS13700 (KHA74_13615) secDF 2803801..2806020 (-) 2220 WP_094032216.1 protein translocase subunit SecDF -
  KHA74_RS13705 (KHA74_13620) - 2806057..2806353 (-) 297 WP_094032215.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87296.70 Da        Isoelectric Point: 4.9498

>NTDB_id=564085 KHA74_RS13685 WP_094032217.1 2800367..2802727(-) (recJ) [Bacillus velezensis strain SW5]
MLASKMRWEIQQPDQEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKMAVSQG
EPIMIYGDYDADGVTSTSVMLTALRQLSADVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCGYPFKELAGVGVAFKLAHALTGSLPEAMLDLAAIGTVADLVPLHDENRLI
ASLGIERLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTEDQFEAEELASDIDRLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVAKAGWNPGVVGIVASKLAERFYRPAVVLGIDEEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVAELRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVESITELNMLSPFGMLN
PKPHVMVKNAVLDDVRLIGANKNHVKMTVKSDSAVLDCVGFNKGELEAGVVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVTALPKEKRVLISFNEKTAQKLRAKELSGELAGITSESEAEALQLDGKYAVFLDVPPSADR
LQHLLDGKKPERIYFVFINHEEHFLSSFPSRNDFKWFYAFLLKRGAFDFGKHGSELARHRGWSIDTINFMTKVFFDLGFV
KIEDGVLSVVSGAKKRDLADSQTYQVKQQLMELDQKLNYSAAYELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=564085 KHA74_RS13685 WP_094032217.1 2800367..2802727(-) (recJ) [Bacillus velezensis strain SW5]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATCAGGAAAAAGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCACTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAAATGGCGGTTTCTCAAGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCGGTGATGCTGACGGCACTGAGACAGCT
GTCGGCGGACGTTGACTTTTATATTCCCGACCGTTTCACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGCTCCATAA
AAGAGCGGGGATTTTCGCTGATCATTACAGTGGATACGGGAATCGCCGCCGTCCATGAAGCTGAAGTCGCAAAAGAGCTG
GGCTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATCATCCACCCGAAGCA
GCCGGGATGCGGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTGGCGTTTAAGCTTGCCCACGCGCTTACGGGTTCTT
TGCCGGAAGCTATGCTGGACTTGGCGGCAATCGGTACGGTTGCAGATCTGGTGCCTCTTCATGATGAAAACAGATTAATT
GCGTCACTCGGAATCGAAAGGCTTCGGACGACAAATCGGCCGGGCCTCAAAGCCCTGATTAAGCTTTCCGGCGGAGATAT
CGGACAAGCTGATGAGGAAACGATCGGATTTCAGGTCGCGCCGAGGCTCAATGCCGTCGGAAGAATAGAACAGGCCGATC
CCGCCGTCCATCTGCTGATGACCGAAGATCAGTTTGAAGCGGAGGAACTGGCTTCGGATATTGACCGGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTTCAGCAGCAAGGCGGAAACCCGTCTGCCATTGT
AGTGGCAAAAGCCGGCTGGAATCCGGGAGTCGTCGGTATTGTCGCATCGAAGCTGGCTGAACGTTTTTACCGTCCCGCTG
TTGTGCTTGGGATAGATGAGGAGCAGGGAATCGCTAAAGGGTCGGCGCGAAGCATCAGAGGCTTCAACCTGTTTACGAGC
CTTTCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGGCATCCGATGGCGGCAGGAATGACATTGAAGGCTGAAGATGT
GGCGGAATTAAGGGACAGGCTGAATCAAATGGCTGAAGAAACCCTTACGGAAGAGGATTTTATTCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTAGAAGACATAACCGTTGAAAGCATTACAGAATTAAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCGCATGTGATGGTGAAAAATGCCGTGCTTGATGATGTGCGCCTGATCGGAGCGAATAAAAATCACGTGAAAAT
GACGGTGAAAAGTGATTCCGCCGTCCTTGACTGCGTCGGCTTTAACAAAGGAGAGCTTGAAGCCGGAGTTGTCCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCCATTAATGAATGGAACAACAGAAAGAAGCCCCAGCTGATGATTAAAGACGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATCAGGGGCAAGCGGACGTGGGAAGACACGGTCACCGCGCTGCCGAAGGAAAA
ACGGGTGCTCATCAGCTTTAACGAAAAAACCGCTCAGAAGCTCCGTGCAAAAGAGCTGTCGGGCGAGCTGGCCGGGATCA
CATCGGAATCCGAGGCGGAAGCTCTCCAGCTTGACGGAAAATATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGATCGC
CTGCAGCATTTGCTTGACGGAAAGAAGCCGGAACGGATTTATTTTGTTTTTATCAATCATGAAGAACATTTCCTGTCCTC
ATTTCCATCTCGAAATGACTTCAAGTGGTTTTACGCATTTCTCTTGAAAAGGGGCGCTTTTGACTTCGGCAAGCATGGTT
CAGAGCTTGCGAGACACAGAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAAGATGGTGTGCTGTCTGTCGTGAGCGGCGCGAAAAAACGCGACCTGGCAGATTCGCAAACCTATCAGGTAAA
ACAGCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCAGCCTATGAGCTGAAGGAATGGCTGAATAAGCTGATGA
ACCAAGGTTCAGAAGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(315-437)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

82.061

100

0.821