Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   L6L55_RS23010 Genome accession   NZ_CP091700
Coordinates   4472366..4473130 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain KFu019     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4467366..4478130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6L55_RS22990 (L6L55_22985) acpT 4468320..4468907 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  L6L55_RS22995 (L6L55_22990) nikA 4469018..4470592 (+) 1575 WP_000953348.1 nickel ABC transporter substrate-binding protein -
  L6L55_RS23000 (L6L55_22995) nikB 4470592..4471536 (+) 945 WP_000947062.1 nickel ABC transporter permease subunit NikB -
  L6L55_RS23005 (L6L55_23000) nikC 4471533..4472366 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  L6L55_RS23010 (L6L55_23005) amiE 4472366..4473130 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  L6L55_RS23015 (L6L55_23010) nikE 4473127..4473933 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  L6L55_RS23020 (L6L55_23015) nikR 4473939..4474340 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=563073 L6L55_RS23010 WP_001136229.1 4472366..4473130(+) (amiE) [Escherichia coli strain KFu019]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=563073 L6L55_RS23010 WP_001136229.1 4472366..4473130(+) (amiE) [Escherichia coli strain KFu019]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
AGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398