Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   L6L66_RS20435 Genome accession   NZ_CP091677
Coordinates   4176568..4177332 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain KFu022     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4171568..4182332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6L66_RS20415 (L6L66_20415) acpT 4172522..4173109 (+) 588 WP_000285791.1 4'-phosphopantetheinyl transferase AcpT -
  L6L66_RS20420 (L6L66_20420) nikA 4173220..4174794 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  L6L66_RS20425 (L6L66_20425) nikB 4174794..4175738 (+) 945 WP_032238187.1 nickel ABC transporter permease subunit NikB -
  L6L66_RS20430 (L6L66_20430) nikC 4175735..4176568 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  L6L66_RS20435 (L6L66_20435) amiE 4176568..4177332 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  L6L66_RS20440 (L6L66_20440) nikE 4177329..4178135 (+) 807 WP_032238188.1 nickel import ATP-binding protein NikE -
  L6L66_RS20445 (L6L66_20445) nikR 4178141..4178542 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=562818 L6L66_RS20435 WP_001136236.1 4176568..4177332(+) (amiE) [Escherichia coli strain KFu022]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=562818 L6L66_RS20435 WP_001136236.1 4176568..4177332(+) (amiE) [Escherichia coli strain KFu022]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398